Systemic distribution of salusin expression in the rat
Systemic distribution of salusin expression in the rat
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DOI：
10.1291/hypres.30.1255
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发表时间：
2007-12-01
影响因子：
5.4
通讯作者：
Hirata, Yukio
中科院分区：
文献类型：
--
作者：
Suzuki, Noriko;Shichiri, Masayoshi;Hirata, Yukio
Salusin-alpha和Salusin-beta是具有消肿和心动过缓作用的多功能生物活性肽。它们最初通过生物信息学分析从全长人类cDNA中鉴定。Salusin肽在mRNA水平上在人体组织中表达，但没有关于其在任何物种中的系统分布的信息。我们研究了preprosalusin mRNA和salusin肽在各种正常大鼠器官中的分布。而preprosalusin mRNA的表达无处不在，免疫反应salusin-beta检测最强烈的下丘脑和垂体后叶，垂体前叶和胃肠道，免疫和造血系统中不太丰富。Salusin-beta阳性细胞似乎是造血或内分泌来源的，许多造血细胞也被抗CD 68染色，抗CD 68特异性识别巨噬细胞。在任何大鼠组织中均未检测到Salusin-α样免疫反应性。这些结果表明，大鼠salusin在免疫学上类似于人salusin-beta，并且广泛表达，特别是在免疫、胃肠道和中枢神经系统中，并且主要在内分泌和造血来源的细胞中。
Salusin-alpha and salusin-beta are multifunctional bioactive peptides with hypotensive and bradycardic effects. They were originally identified from full-length human cDNAs by bioinformatics analyses. Salusin peptides are expressed in human tissues at the mRNA level, but no information is available about their systemic distributions in any species. We examined the distributions of preprosalusin mRNA and the salusin peptides in a variety of normal rat organs. Whereas preprosalusin mRNA was expressed ubiquitously, immunoreactive salusin-beta was detected most strongly in the hypothalamus and posterior pituitary, and less abundantly in the anterior pituitary and gastrointestinal, immune, and hematopoietic systems. Salusin-beta-positive cells appeared to be of either hematopoietic or endocrine origin, and many hematopoietic cells were also stained with anti-CD68, which specifically recognizes macrophages. Salusin-alpha-like immunoreactivity was not detected in any of the rat tissues. These results indicate that rat salusin is immunologically similar to human salusin-beta and widely expressed, especially in the immune, gastrointestinal, and central nervous systems and mainly in endocrine- and hematopoietic-derived cells.