Prevalence and transmission of extensively drug-resistant Salmonella enterica serovar Kentucky ST198 based on whole-genome sequence in an intensive laying hen farm in Jiangsu, China
Prevalence and transmission of extensively drug-resistant Salmonella enterica serovar Kentucky ST198 based on whole-genome sequence in an intensive laying hen farm in Jiangsu, China
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DOI：
10.1016/j.psj.2024.103608
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发表时间：
2024-03-29
期刊：
影响因子：
4.4
通讯作者：
Pan，Zhiming
中科院分区：
文献类型：
--
作者：
Liu，Bowen;Meng，Chuang;Pan，Zhiming
沙门氏菌在自然界中广泛分布，是一种重要的人畜共患病原菌，不仅阻碍了畜禽相关产业的发展，而且对人类健康构成了极大的威胁.本研究从2020年至2023年（每年1 - 2个月）采集弱鸡、死胚、粪便和环境样品1,537份，对集约化蛋鸡场沙门氏菌的流行情况进行长期监测，共分离沙门氏菌105株，分离率为6.83%（105/1,537）。随时间推移，沙门氏菌感染率明显下降（P< 0.001）。2020年以前，优势血清型为S。肠炎2020年11月首次检出肯塔基州，逐渐发现其比例超过S.从那时起就一直在肠道。肯塔基州分离株分布于鸡场四个区域的各个环节。根据全基因组测序结果，共将55株肯塔基沙门氏菌归属于ST 198。其中54株对12 ~ 16种抗生素耐药，为广泛耐药（XDR）。在55株肯塔基沙门氏菌中检测到17种耐药基因。大多数菌株的耐药表型与其基因型一致。2020年至2023年从该农场分离的所有肯塔基州沙门氏菌菌株均显示出基于核心基因组SNP的同源性较高。溯源分析表明，S.肯塔基州是通过新购买的羊群引入农场的。XDRS的长期存在。肯塔基州ST 198造成了很大的风险，因为在这个家禽农场的多年龄管理和工人的流通。因此，这项研究是第一个报告广泛耐药。中国这家集约化养鸡场检出肯塔基州ST 198。
Salmonella, which is widely distributed in nature, is an important zoonotic pathogen affecting humans, livestock, and other animals.Salmonellainfection not only hinders the development of livestock and poultry-related industries but also poses a great threat to human health. In this study, we collected 1,537 samples including weak chicks, dead embryos, fecal samples and environmental samples from 2020 to 2023 (for a period of 1 to 2 months per year) to keep a long-term monitor the prevalence ofSalmonellain an intensive laying hen farm, 105Salmonellastrains were isolated with an isolation rate of 6.83% (105/1,537). It revealed a significant decrease in prevalence rates ofSalmonellaover time (P< 0.001). Before 2020, the predominant serotype wasS. Enteritidis.S. Kentucky was first detected in November 2020 and its proportion was gradually found to exceed that ofS. Enteritidis since then.S. Kentucky isolates were distributed in various links of the four regions in the poultry farm. A total of 55S.Kentucky strains, were assigned to ST198 based on whole genome sequencing. Among them, 54 strains were resistant to 12 to 16 antibiotics, indicating that they were extensively drug-resistant (XDR). Seventeen antimicrobial resistance genes were detected in 55S.Kentucky isolates. For most of these isolates, antibiotic resistance phenotypes were concordant with their genotypes. AllS.Kentucky strains isolated from this farm in 2020 to 2023 showed a high similarity based on their core-genome SNP-based phylogeny. The traceability analysis revealed thatS. Kentucky was introduced to the farm through newly purchased flocks. The long-term existence of XDRS. Kentucky ST198 poses a substantial risk because of the multiage management and circulation of workers in this poultry farm. Thus, this study is the first to report extensively drug-resistantS. Kentucky ST198 detected in this intensive poultry farm in China.