INVESTIGATING THE SOURCE OF AMNIOTIC-FLUID ISOLATES OF FUSOBACTERIA
INVESTIGATING THE SOURCE OF AMNIOTIC-FLUID ISOLATES OF FUSOBACTERIA
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DOI：
10.1093/clinids/16.supplement_4.s423
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发表时间：
1993-06-01
影响因子：
11.8
通讯作者：
HILL, GB
中科院分区：
文献类型：
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作者：
HILL, GB
菌株的重新鉴定结果显示，从羊水与下生殖道分离的种和亚种模式存在一些统计学显著差异（表1）。F.具核梭杆菌（所有亚种）占从羊水中分离的菌株的83%，仅占从下生殖道中分离的菌株的31%;相反，从这些部位分离的菌株中，naviforme梭杆菌分别占0%和42%（表1）。从羊水中分离的梭菌属的种和亚种与从健康和患病龈下部位培养的梭菌属的种和亚种最相似：在这些部位，F. nucleatum亚种vincentii最常分离，其次是F. nucleatum subspecies nucleatum和F. nucleatum subspecies polymorphum，而F. naviforme和Fusobacteriumgonidiaformans分别很少或从未分离（个人交流，Lillian和WEC摩尔）。在我们的研究中，F. naviforme是最常从下生殖道分离的菌种，但在羊水中未发现。然而，由于F. nucleatum subspecies vincentii和F.在下生殖道中也发现有核菌亚种，尽管不太常见（表1）。但是，这些结果应谨慎解释，因为从下生殖道获得的分离株来自非妊娠女性，妊娠的生理状态和/或性习惯或其他行为的变化可能影响生殖器定植。此外，未怀孕妇女的中位年龄为28岁，而怀孕妇女的中位年龄为22岁。妊娠和非妊娠女性的种族分布没有差异（约三分之一的白人和三分之二的黑人）。以下是12份含有梭菌的羊水样本的其他参数和这些患者的临床结局（从病历审查中获得）。每毫升羊水中梭杆菌的活菌数从几个到几百万个不等。在其中四种羊水中有伴生微生物，即拟杆菌
The results of reidentification of strains revealed some statistically significant differences in the patterns of species and subspecies isolated from the amniotic fluid vs. the lower genital tract (table 1). F. nucleatum (all subspecies) represented 83% of strains isolated from amniotic fluid and only 31% of the strains isolated from the lower genital tract; in contrast, Fusobacterium naviforme represented 0% and 42%, respectively, of strains isolated from these sites (table 1). The species and subspecies of fusobacteria isolated from amniotic fluid (table 1) were most similar to species and subspecies of fusobacteria cultured from healthy and diseased subgingival sites: in these sites, F. nucleatum subspecies vincentii is isolated most often, followed by F. nucleatum subspecies nucleatum and F. nucleatum subspecies polymorphum, whereas F. naviforme and Fusobacterium gonidiaformans, respectively, are rarely or never isolated (personal communication, Lillian and WEC Moore). In our study, F. naviforme, the species most commonly isolated from the lower genital tract, was not found in amniotic fluid. However, the source of the amniotic isolates was not conclusively determined because F. nucleatum subspecies vincentii and F. nucleatum subspecies nucleatum were also found, albeit less commonly, in the lower genital tract (table 1).These results, however, should be interpreted with caution because the isolates obtained from the lower genital tract were from nonpregnant women and the physiological state of pregnancy and/or changes in sexual habits or other behavior might influence genital colonization. In addition, the median age for the nonpregnant women was 28 years, whereas that for the pregnant women was 22 years. The racial distributions for the pregnant and nonpregnant women were not different (approximately one-third Caucasian and two-thirds black). The following are additional parameters of the 12 samples of amniotic fluid that contained fusobacteria and the clinical outcomes (obtained from a review of medical charts) for these patients. The viable counts of fusobacteria ranged from only a few to millions per milliliter of amniotic fluid. In four of these amniotic fluids there were accompanying organisms, ie, Bacteroides