Secondary Intracellular Symbiotic Bacteria in Aphids of the GenusYamatocallis (Homoptera: Aphididae: Drepanosiphinae)
Secondary Intracellular Symbiotic Bacteria in Aphids of the GenusYamatocallis (Homoptera: Aphididae: Drepanosiphinae)
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大马蚜属（同翅目：蚜科：Drepanosiphinae）的次生细胞内共生细菌
DOI：
10.1128/aem.67.11.5315-5320.2001
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发表时间：
2001
影响因子：
4.4
通讯作者：
T. Fukatsu
中科院分区：
文献类型：
--
作者：
T. Fukatsu
摘要利用分子系统发育分析、原位杂交和诊断PCR技术，对一株新的蚜虫胞内共生细菌Yamatocallis进行了鉴定。在蚜虫组织中，这种细菌（暂命名为YSMS [Yamatocallis次级菌胞共生体]）被发现专门在大细胞包围的初级菌胞窝藏Buchnera细胞。在检查的9种螺旋管蚜中，仅在同一属的两个物种Yamatocallis tokyoensis和Yamatocallis hirayamae中检测到YSMS。在这些蚜虫的自然种群中，YSMS存在于100%的个体中。基于16S rDNA序列的系统发育分析表明，Y. tokyoensis和Y. hirayamae构成变形菌纲γ亚门中一个独特而孤立的分支。没有16S rDNA序列的次级内共生体的特点，到目前为止，从其他蚜虫表现出系统发育的亲和力YSMS。基于这些结果，我认为YSMS是由Yamatocalli属的祖先获得的，并在整个谱系的进化过程中一直保持不变。利用Buchnera spp.的16S rDNA核苷酸替换率，YSMS的获得时间估计为大约1300万至2600万年前，在第三纪的中新世。
ABSTRACT A novel secondary intracellular symbiotic bacterium from aphids of the genus Yamatocallis (subfamily Drepanosiphinae) was characterized by using molecular phylogenetic analysis, in situ hybridization, and diagnostic PCR detection. In the aphid tissues, this bacterium (tentatively designated YSMS [Yamatocallis secondary mycetocyte symbiont]) was found specifically in large cells surrounded by primary mycetocytes harboringBuchnera cells. Of nine drepanosiphine aphids examined, YSMS was detected in only two species of the same genus,Yamatocallis tokyoensis and Yamatocallis hirayamae. In natural populations of these aphids, YSMS was present in 100% of the individuals. Phylogenetic analysis based on 16S ribosomal DNA (rDNA) sequences demonstrated that YSMS ofY. tokyoensis and Y. hirayamae constitute a distinct and isolated clade in the γ subdivision of the classProteobacteria. No 16S rDNA sequences of secondary endosymbionts characterized so far from other aphids showed phylogenetic affinity to YSMS. Based on these results, I suggest that YSMS was acquired by an ancestor of the genus Yamatocallisand has been conserved throughout the evolution of the lineage. By using the nucleotide substitution rate for 16S rDNA ofBuchnera spp., the time of acquisition of YSMS was estimated to be about 13 to 26 million years ago, in the Miocene epoch of the Tertiary period.