A novel transcription factor negatively regulates antioxidant response, cell wall integrity and virulence in the fungal insect pathogen, Beauveria bassiana.
A novel transcription factor negatively regulates antioxidant response, cell wall integrity and virulence in the fungal insect pathogen, Beauveria bassiana.
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DOI：
10.1111/1462-2920.15397
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发表时间：
2021-01
影响因子：
5.1
通讯作者：
Xin Zhao;Tingying Luo;Shuaishuai Huang;N. Peng;Ying Yin;Zhibing Luo;Yongjun Zhang
中科院分区：
文献类型：
--
作者：
Xin Zhao;Tingying Luo;Shuaishuai Huang;N. Peng;Ying Yin;Zhibing Luo;Yongjun Zhang
基因组数据已经确定了一类真菌特异性转录因子（FsTFs），它们被认为调节真菌基因表达的独特方面，尽管其中许多蛋白质的功能尚不清楚。本文在丝状昆虫真菌白僵菌（Beauveria bassiana）中鉴定了一种新的FsTF (BbStf1)，该FsTF具有亮氨酸拉链二聚化结构域和真菌转录因子调控的中间同源区。实验证实了BbStf1的转录激活和核定位。破坏Bbstf1导致对氧化应激和细胞壁扰动的耐受性增加，并伴有过氧化物酶（POD）和超氧化物歧化酶（SOD）活性增加，还原性/氧化性谷胱甘肽（GSH/GSSG）比例增加，细胞壁增厚和组成改变。基因表达谱分析显示，抗氧化酶和细胞壁完整性相关基因的转录模式在∆Bbstf1中发生了改变，其中一些Bbstf1靶向基因得到了明确的证据。与野生型菌株相比，∆bstf1菌株通过局部感染和血内注射对mellonella Galleria表现出更大的毒力，这是因为它在血中的增殖速度更快。总之，BbStf1在球孢白僵菌的抗氧化反应、细胞壁完整性和毒力中起负调节作用。这篇文章受版权保护。版权所有。
Genomic data have identified a class of fungal specific transcription factors (FsTFs) that are thought to regulate unique aspects of fungal gene expression, although the functions of many of these proteins remains unknown. Here, a novel FsTF (BbStf1), which features a leucine zipper dimerization domain and a fungal transcription factor regulatory middle homology region, was characterized in Beauveria bassiana, a filamentous insect fungal pathogen. Transcriptional activation and nuclear localization were experimentally confirmed for BbStf1. Disruption of Bbstf1 resulted in increased tolerance to oxidative stress and cell wall perturbation, accompanied by increased peroxidase (POD) and superoxide dismutase (SOD) activities and ratio of reduced/ oxidized glutathione (GSH/GSSG), and by thickened cell wall and altered composition. Gene expression profile analysis revealed that transcription patterns of antioxidant enzyme and cell wall integrity-involved genes were altered in the ∆Bbstf1, including some BbStf1-targeted genes clarified with evidence. The ∆Bbstf1 strain displayed greater virulence to Galleria mellonella in the bioassays through both topical infection and intrahemocoel injection due to more rapid proliferation in the hemocoel as compared to the wild-type strain. Altogether, BbStf1 acts as a negative regulator of antioxidant response, cell wall integrity and virulence in B. bassiana. This article is protected by copyright. All rights reserved.