Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation
Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation
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DOI：
10.1007/s00705-014-2072-9
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发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Lee, Sukchan
中科院分区：
文献类型：
--
作者：
Kil, Eui-Joon;Byun, Hee-Seong;Lee, Sukchan
番茄黄化卷叶病毒(TYLCV)是番茄黄化卷叶病毒属的成员，基因组为单链DNA。TYLCV可在番茄上引起严重的病害症状，但其他寄主植物如葫芦和辣椒则没有症状。从侵染韩国TYLCV的番茄中用滚环扩增法构建了一个韩国TYLCV分离物的全长DNA克隆。为评价甜椒品种对TYLCV的相对感病能力，采用田间接种的方法，对19个甜椒品种进行了TYLCV的接种。所有被TYLCV感染的甜椒都没有症状，尽管Southern杂交和聚合酶链式反应分析表明TYLCV基因组DNA在根、茎和新生的新梢中积累。Southern杂交结果表明，甜椒TYLCV从接种后的顶端向根或新梢有系统地复制和迁移。粉虱介导法接种试验表明，TYLCV可以从侵染TYLCV的甜椒上传播到番茄上。综上所述，这些结果表明，甜椒在自然界中可能是TYLCV的储存库。
Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, has a single-stranded DNA genome. TYLCV can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. A full-length DNA clone of a Korean TYLCV isolate was constructed by rolling-circle amplification from TYLCV-infected tomatoes in Korea. To assess relative susceptibility of sweet pepper varieties to TYLCV, 19 cultivars were inoculated with cloned TYLCV by agro-inoculation. All TYLCV-infected sweet peppers were asymptomatic, even though Southern hybridization and polymerase chain reaction analysis showed TYLCV genomic DNA accumulation in roots, stems, and newly produced shoots. Southern hybridization indicated that TYLCV replicated and moved systemically from agro-inoculated apical shoot tips to roots or newly produced shoots of sweet peppers. Whitefly-mediated inoculation experiments showed that TYLCV can be transmitted to tomatoes from TYLCV-infected sweet peppers. Taken together, these results indicate that sweet pepper can be a reservoir for TYLCV in nature.