Role of gut flora on intestinal group II phospholipase A2 activity and intestinal injury in shock
Role of gut flora on intestinal group II phospholipase A2 activity and intestinal injury in shock
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DOI：
10.1152/ajpgi.2001.281.4.g957
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发表时间：
2001-10-01
影响因子：
4.5
通讯作者：
Hsueh, W
中科院分区：
文献类型：
--
作者：
Rozenfeld, RA;Liu, XL;Hsueh, W
我们之前的研究表明，II组磷脂酶A(2) （PLA(2)-II）是肠隐窝中的一种分泌性、杀菌性和促炎蛋白，在脂多糖（LPS）和血小板活化因子（PAF）刺激后上调。在这里，我们研究了无菌环境（GF）或抗生素治疗（ABX）是否会影响PAF （1.5 mg/kg）或LPS （8 mg/kg）注射后的病理生理反应和肠道PLA(2)-II活性。我们发现LPS和PAF可引起常规喂养（CN）大鼠的低血压和轻度肠道损伤；这些变化在ABX大鼠中较轻，而GF大鼠未出现肠道损伤。检测正常大鼠小肠中聚乳酸(2)-II酶活性；ABX和GF大鼠的基础水平没有降低。PAF和LPS引起PLA(2)-II活性升高，而在GF和ABX大鼠中没有这种作用。肠内污染对GF大鼠的再定殖恢复了它们对PAF和LPS的PLA(2)-II反应和对肠损伤的易感性。我们得出结论，PAF和lps诱导的PLA(2)- ii活性的增加依赖于肠道细菌，ABX和GF大鼠比CN大鼠更不容易受到lps诱导的损伤。
We previously showed that group II phospholipase A(2) (PLA(2)-II), a secretory, bactericidal, and proinflammatory protein in intestinal crypts, is upregulated after lipopolysaccharide (LPS) and platelet-activating factor (PAF) challenge. Here we examined whether germ-free environment (GF) or antibiotic treatment (ABX) affects the pathophysiological responses and intestinal PLA(2)-II activity after PAF (1.5 mug/kg) or LPS (8 mg/kg) injection. We found that LPS and PAF induced hypotension and mild intestinal injury in conventionally fed (CN) rats; these changes were milder in ABX rats, whereas GF rats showed no intestinal injury. PLA(2)-II enzyme activity was detected in normal rat small intestine; the basal level was not diminished in ABX or GF rats. PAF and LPS caused an increase in PLA(2)-II activity, which was abrogated in GF and ABX rats. Recolonization of GF rats by enteral contamination restituted their PLA(2)-II response to PAF and LPS and susceptibility to bowel injury. We conclude that PAF- and LPS-induced increases in PLA(2)-II activity are dependent on gut bacteria, and ABX and GF rats are less susceptible to LPS-induced injury than CN rats.