Sfp1 is a stress- and nutrient-sensitive regulator of ribosomal protein gene expression
Sfp1 is a stress- and nutrient-sensitive regulator of ribosomal protein gene expression
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DOI：
10.1073/pnas.0405353101
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发表时间：
2004-10-05
影响因子：
11.1
通讯作者：
O'Shea, EK
中科院分区：
文献类型：
--
作者：
Marion, RM;Regev, A;O'Shea, EK
酵母细胞通过对核糖体蛋白（RP）基因的转录调控，根据生理需求调节其蛋白质合成能力。在此我们证明，转录因子Sfp1（先前已表明在细胞大小的控制中起作用）会根据营养物质和应激情况调节RP基因的表达。在最佳生长条件下，Sfp1定位于细胞核，结合到RP基因的启动子上，并有助于促进RP基因的表达。当雷帕霉素靶蛋白（TOR）信号传导受到抑制、出现应激情况或营养物质可利用性发生变化时，Sfp1从RP基因启动子上释放并离开细胞核，RP基因转录被下调。此外，缺乏Sfp1的细胞无法根据环境信号适当地调节RP基因的表达。我们得出结论，Sfp1整合了来自营养物质和应激响应信号通路的信息，以帮助控制RP基因的表达。
Yeast cells modulate their protein synthesis capacity in response to physiological needs through the transcriptional control of ribosomal protein (RP) genes. Here we demonstrate that the transcription factor Sfp1, previously shown to play a role in the control of cell size, regulates RIP gene expression in response to nutrients and stress. Under optimal growth conditions, Sfp1 is localized to the nucleus, bound to the promoters of RP genes, and helps promote RP gene expression. In response to inhibition of target of rapamycin (TOR) signaling, stress, or changes in nutrient availability, Sfp1 is released from RP gene promoters and leaves the nucleus, and RP gene transcription is down-regulated. Additionally, cells lacking Sfp1 fail to appropriately modulate RIP gene expression in response to environmental cues. We conclude that Sfp1 integrates information from nutrient- and stress-responsive signaling pathways to help control RP gene expression.