A MICROANALYTICAL PROCEDURE FOR THE DETERMINATION OF CALCIUM, PHOSPHATE AND FLUORIDE IN ENAMEL BIOPSY SAMPLES
A MICROANALYTICAL PROCEDURE FOR THE DETERMINATION OF CALCIUM, PHOSPHATE AND FLUORIDE IN ENAMEL BIOPSY SAMPLES
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DOI：
10.1159/000260645
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发表时间：
1983-01-01
期刊：
影响因子：
4.2
通讯作者：
BROWN, WE
中科院分区：
文献类型：
--
作者：
VOGEL, GL;CHOW, LC;BROWN, WE
本文介绍了用酸蚀法从大鼠和人牙釉质中连续层中测定钙、磷和氟的分析方法。这些方法包括：（i）用于测定钙和磷酸盐的简单、灵敏的分光光度法;（ii）易于构造的微型氟化物电极;以及（iii）改进的电极适配器，其允许一个氟化物电极非常快速地测量许多微量样品。所描述的分析技术的高灵敏度和简单性允许以迄今为止仅用大得多的样品才能达到的精度和速度分析牙科研究中感兴趣的许多类型的微量样品。
This paper describes analytical methods for the determination of calcium, phosphate and fluoride in successive layers of enamel removed from rat and human teeth by acid etching. These methods include: (i) simple, sensitive spectrophotometric methods for the determination of calcium and phosphate; (ii) an easily constructed miniature fluoride electrode, and (iii) an improved electrode adapter that permits one fluoride electrode to measure numerous microsamples very rapidly. The high sensitivity and simplicity of the analytical techniques described allows many types of microsamples of interest in dental research to be analyzed with an accuracy and speed heretofore attainable only with much larger samples.