Induction of immunomodulating cytokines by a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes
Induction of immunomodulating cytokines by a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes
复制标题
DOI：
10.1016/s0162-3109(98)00043-5
复制
发表时间：
1998-11-01
期刊：
影响因子：
--
通讯作者：
Gao, Y
中科院分区：
文献类型：
--
作者：
Liu, MQ;Li, JZ;Gao, Y
研究了一种新的香菇菌丝体多糖肽复合物（LE）的免疫调节作用，以阐明其增强细胞免疫的机制。从未处理和处理的鼠脾细胞和人外周血单核细胞中分离RNA样品。RT-PCR检测细胞因子基因的表达，生物测定法检测细胞因子的产生。通过给予LE，IL-2和TNF-α基因在处理的小鼠脾单个核细胞和人外周血单个核细胞中的表达水平增加。IL-2的产生在经处理的小鼠脾单核细胞中增加，并且TNF-α的产生在经处理的小鼠腹腔渗出液巨噬细胞中增加。IL 2和TNF-α的产生在处理的人外周血单核细胞中增加。提示LE可诱导Th免疫应答。(C)1998 Elsevier Science B. V.保留所有权利。
The immunomodulating effect of a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes (LE) was studied for elucidation of the mechanism of augmentation of cell-mediated immunity. RNA samples were isolated from the untreated and treated murine splenocytes and human peripheral blood mononuclear cells. RT-PCR was used to analyze the cytokine gene expression and bioassay was used to analyze the cytokine production. By administration of LE, the expression levels of IL-2 and TNF-alpha genes were augmented in the treated murine spleen mononuclear cells and human peripheral blood mononuclear cells. The production of IL-2 were augmented in the treated murine spleen mononuclear cells, and the production of TNF-alpha were augmented in the treated murine peritoneal exudate macrophages. The production of IL2 and TNF-alpha were augmented in the treated human peripheral blood mononuclear cells. These results suggest that LE may induce Th immune responses. (C) 1998 Elsevier Science B.V. All rights reserved.