Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus
Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus
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DOI：
10.1099/vir.0.80247-0
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发表时间：
2004-12-01
影响因子：
3.8
通讯作者：
Takashima, I
中科院分区：
文献类型：
--
作者：
Shirato, K;Miyoshi, H;Takashima, I
对西尼罗病毒（WN）的两个纽约（NY）毒株进行了空斑纯化，分离出包膜(E)蛋白序列中n -链糖基化位点上氨基酸序列不同的4个变异株。在这些菌株变体中，只有两种E蛋白被糖基化。为了确定E蛋白糖基化与WIN病毒致病性之间的关系，6周大的小鼠皮下感染了这些变体。感染携带糖基化E蛋白的病毒的小鼠发生致命感染，而感染携带非糖基化E蛋白的病毒的小鼠死亡率较低。相反，携带E蛋白糖基化或非糖基化形式的病毒在小鼠脑内感染导致致命感染。这些结果表明，E蛋白糖基化是WN病毒NY株神经侵袭性的分子决定因素。
Two New York (NY) strains of the West Nile (WN) virus were plaque-purified and four variants that had different amino acid sequences at the N-linked glycosylation site in the envelope (E) protein sequence were isolated. The E protein was glycosylated in only two of these strain variants. To determine the relationship between E protein glycosylation and pathogenicity of the WIN virus, 6-week-old mice were infected subcutaneously with these variants. Mice infected with viruses that carried the glycosylated E protein developed lethal infection, whereas mice infected with viruses that carried the non-glycosylated E protein showed low mortality. In contrast, intracerebral infection of mice with viruses carrying either the glycosylated or non-glycosylated forms of the E protein resulted in lethal infection. These results suggested that E protein glycosylation is a molecular determinant of neuroinvasiveness in the NY strains of WN virus.