Molecular and cellular mechanisms of cadmium resistance in cultured cells.
Molecular and cellular mechanisms of cadmium resistance in cultured cells.
复制标题
培养细胞耐镉的分子和细胞机制。
DOI：
10.1007/978-3-0348-6784-9_44
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
C. Hildebrand
中科院分区：
文献类型：
--
作者：
D. Grady;R. Moyzis;C. Hildebrand
重金属诱导金属硫蛋白（MTs）的合成为基因表达的基础机制研究提供了一个理想的模型系统。细胞系在其抗重金属已被分离通过暴露于连续增加的镉水平，然后通过克隆分离的制度。这些细胞系已被用于检查甲基化和扩增在镉抗性（Cdr）表型中的作用。这表明MT基因在Cdr中国仓鼠细胞中的表达调控在转录和翻译水平上都受到调节。对MT2基因序列的分析揭示了第一内含子中的潜在可变剪接位点。该位点的使用将在氨基酸9和10之间插入3或12个额外的氨基酸。对培养细胞中MT2基因转录本的剪接模式的分析表明，第二个内含子在第一个内含子切除之前被优先去除。
Heavy metal induction of the synthesis of metallothioneins (MTs) provides an ideal model system for basic mechanistic studies of gene expression. Cell lines varying in their resistance to heavy metals have been isolated through a regime of exposure to serially increasing levels of Cd followed by clonal isolation. These cell lines have been used to examine the role of methylation and amplification in the Cd-resistant (Cdr) phenotype. It is suggested that regulation of expression of the MT genes in Cdr Chinese hamster cells is modulated at both the transcriptional and translational levels. An analysis of the MT2 gene sequence has uncovered a potential alternative splice site in the first intron. Usage of this site would insert 3 or 12 additional amino acids between amino acids 9 and 10. Analysis of the splicing pattern of the MT2 gene transcript in cultured cells has indicated that the second intron is preferentially removed prior to first intron excision.
大鼠肝、肾谷胱甘肽和金属硫蛋白水平的关系。
影响因子：
4.5
作者：
Wong,KL;Klaassen,CD
通讯作者：
Klaassen,CD