Cloning, molecular characterization of a 13-kDa antigen from Schistosoma japonicum, Sj13, a putative salivary diagnosis candidate for Schistosomiasis japonica
Cloning, molecular characterization of a 13-kDa antigen from Schistosoma japonicum, Sj13, a putative salivary diagnosis candidate for Schistosomiasis japonica
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日本血吸虫 Sj13 的 13 kDa 抗原的克隆和分子表征，Sj13 是日本血吸虫病的假定唾液诊断候选者
DOI：
10.1007/s00436-009-1575-x
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发表时间：
2009-07
影响因子：
2
通讯作者：
Yang, Lin-lin
中科院分区：
文献类型：
--
作者：
Zheng, Huan-qin;Lv, Zhi-yue;You, Xu;Yu, Xin-bing;Hu, Wei;Wu, Zhong-dao;Sun, Xi;Zhou, Yan-ping;Yang, Lin-lin
Saliva has been suggested as an easily accessible and a noninvasive diagnostic alternative for detection of antibodies. To identify and characterize Schistosoma japonicum (S. japonicum) antigens that are recognized by saliva of infected host, we have used a pool of saliva from infected patients to immunoscreen an egg cDNA library of S. japonicum. The open reading frame of the isolated two clones encodes same protein of 116 amino acids exhibiting 100% identity to an amino acid sequence (AY222893) of S. japonicum in NCBInr database. The protein encoded is inferred a secretory protein with a molecular mass of 13 kDa (Sj13) and shares no homology to any entries in the NCBInr database, demonstrating that Sj13 might be a schistosome-specific protein. Reverse transcriptase polymerase chain reaction, Western blotting, and immunolocalization analysis revealed Sj13 could be detected in cercaria, adult, and egg and was localized to forehead and tegument of cercaria, cell body (“cytons”) of adult worm, egg shell, and epidermal plate of miracidium. Furthermore, Sj13 showed a good antigenicity when reacted with saliva or serum from schistosomiasis patients. The recombinant Sj13 (rSj13) expressed and purified from Escherichia coli was applied to detect its specific salivary antibody for schistosomiasis diagnosis by an indirect enzyme linked immunosorbent assay (ELISA). Preliminary laboratory test of 116 subjects, 40 with parasitologically proven S. japonicumm infection, 46 with other infectious diseases, and 30 negative controls exhibited 92.50% sensitivity with saliva/rSj13 and 95.00% with serum/SWAP (P > 0.05). The specificity of the ELISA using saliva/rSj13 was 92.11% versus 85.53% with serum/SWAP (P < 0.05). No direct correlations of anti-Sj13 IgG levels with egg counts in stool were observed in saliva detection. These results suggest that Sj13 specific salivary antibody detection may be useful as an antigen for the salivary diagnosis of schistosomiasis japonica and contribute to epidemiological study of schistosomiasis infection in endemic areas.
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影响因子：
--
作者：
D. Malamud;H. Bau;S. Niedbala;P. Corstjens
通讯作者：
D. Malamud;H. Bau;S. Niedbala;P. Corstjens
影响因子：
2.7
作者：
R. T. Pinho;R. Pedrosa;P. Costa-Martins;L. Castello-Branco
通讯作者：
R. T. Pinho;R. Pedrosa;P. Costa-Martins;L. Castello-Branco
影响因子：
3.1
作者：
G. Long;R. Lewert;R. Pelley
通讯作者：
G. Long;R. Lewert;R. Pelley
影响因子：
--
作者：
Malamud D
通讯作者：
Malamud D
影响因子：
8.8
作者：
Lazzarotto, Tiziana;Guerra, Brunella;Landini, Maria Paola
通讯作者：
Landini, Maria Paola