PHORBOL MYRISTIC ACETATE ENHANCES THE PRODUCTION OF INTERLEUKIN-2
PHORBOL MYRISTIC ACETATE ENHANCES THE PRODUCTION OF INTERLEUKIN-2
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DOI：
10.1016/0008-8749(81)90157-x
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发表时间：
1981-01-01
影响因子：
4.3
通讯作者：
FARRAR, JJ
中科院分区：
文献类型：
--
作者：
FULLERFARRAR, J;HILFIKER, ML;FARRAR, JJ
白介素[IL] 2是由cona激活的小鼠脾细胞产生的抗原非特异性因子，具有多种生物活性，包括刺激胸腺细胞增殖、支持连续T细胞系生长、增强裸小鼠脾细胞的抗体反应和支持抗原特异性细胞毒性T细胞的产生。为了获得更多的IL - 2用于进一步的纯化和生物学研究，研究了使用phorbol myristic acetate （PMA）作为共刺激剂。在Con - a诱导的小鼠脾细胞培养中添加PMA，在含血清和无血清培养条件下，IL - 2活性的产生增加了5- 20倍。
Interleukin [IL] 2 is an antigen-nonspecific factor produced by concanavalin A (Con A)-activated mouse spleen cells that has a number of biologic activities including the capacity to stimulate thymocyte proliferation, support continuous T cell line growth, augment the antibody response of nude mouse spleen cells and support the generation of antigen-specific cytotoxic T cells. To obtain increased amounts of IL 2 for the further purification and biologic studies, the use of phorbol myristic acetate (PMA) as a costimulant was studied. The addition of PMA to Con A-induced mouse spleen cell cultures results in a 5- to 20-fold increase in IL 2 activity production under serum-containing and serum-free culture conditions.