A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts.
A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts.
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正常小鼠成纤维细胞中的生长反应基因（16C8）与具有红细胞增强活性的人胶原酶抑制剂同源：诱导型和组成型转录物的证据。
DOI：
10.1093/nar/14.22.8863
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发表时间：
1986
影响因子：
14.9
通讯作者：
D. T. Denhardt
中科院分区：
文献类型：
--
作者：
Dylan R. Edwards;Paul Waterhouse;Martha L. Holman;D. T. Denhardt
我们提出了一个基本上全长的cDNA克隆16 C8的DNA序列，16 C8是从小鼠胚胎成纤维细胞cDNA文库中分离的生长因子诱导基因。0.9 kb的mRNA编码一个Mr为22，500的蛋白质，该蛋白质与一种人类蛋白质具有很大的同源性，据报道，该蛋白质具有增强红细胞分化和抑制胶原酶和其他组织金属蛋白酶的能力。预测的蛋白质的N-末端具有分泌蛋白质的疏水性质特征，并且存在两个N-连接糖基化的潜在位点。16 C8 mRNA的细胞质浓度在静止成纤维细胞的血清刺激后约6小时的中期G1达到最大。北方印迹分析显示，随着血清刺激后时间的增加，诱导的16 C8转录物的大小逐渐减少。这表明是由于聚（A）尾部长度减少所致。mRNA的5'部分的S1分析揭示了三种不同种类的转录物的存在，其中只有一种是可诱导的。
We present the DNA sequence of an essentially full-length cDNA clone of 16C8, a growth factor-inducible gene isolated from a mouse embryo fibroblast cDNA library. The 0.9-kb mRNA encodes an Mr 22,500 protein that has substantial homology to a human protein with the reported abilities to potentiate erythroid differentiation and to inhibit collagenases and other tissue metalloproteinases. The N-terminus of the predicted protein has a hydrophobic nature characteristic of secreted proteins, and two potential sites for N-linked glycosylation are present. The cytoplasmic concentration of 16C8 mRNA is maximal in mid G1 at about 6 h after serum stimulation of quiescent fibroblasts. Northern blot analysis showed a progressive reduction in the size of the induced 16C8 transcripts with increasing time after serum stimulation. This was shown to be due to the reduction in length of the poly(A) tails. S1 analysis of the 5' portion of the mRNA revealed the presence of three different species of transcript, only one of which was inducible.