Alterations in the developing testis transcriptome following embryonic vinclozolin exposure
Alterations in the developing testis transcriptome following embryonic vinclozolin exposure
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DOI：
10.1016/j.reprotox.2010.05.086
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发表时间：
2010-11-01
影响因子：
3.3
通讯作者：
Skinner, Michael K.
中科院分区：
文献类型：
--
作者：
Clement, Tracy M.;Savenkova, Marina I.;Skinner, Michael K.
本研究探讨宫内暴露长春花碱对F1代大鼠睾丸转录组发育的直接影响。以前的研究已经证明，在胚胎性腺性别决定期间暴露于长春新灵会诱导生殖系的表观遗传学改变和跨代成人发病状态。基因芯片分析比较了对照和长春新碱处理的胚胎第13、14和16天的睾丸转录。共鉴定了576个差异表达基因，并检测了与这些改变相关的主要细胞功能和途径。不同发育时期的调控基因集被发现是瞬时改变和不同的。通过改变基因的主要已知功能进行分类。特定的细胞过程和途径分析表明，Wnt和钙信号、血管发育和表观遗传机制是长春花碱直接产生F1作用的潜在中介。(C)2010 Elsevier Inc.保留所有权利。
The current study investigates the direct effects of in utero vinclozolin exposure on the developing F1 generation rat testis transcriptome. Previous studies have demonstrated that exposure to vinclozolin during embryonic gonadal sex determination induces epigenetic modifications of the germ line and transgenerational adult onset disease states. Microarray analyses were performed to compare control and vinclozolin treated testis transcriptomes at embryonic days 13,14 and 16. A total of 576 differentially expressed genes were identified and the major cellular functions and pathways associated with these altered transcripts were examined. The sets of regulated genes at the different development periods were found to be transiently altered and distinct. Categorization by major known functions of altered genes was performed. Specific cellular process and pathway analyses suggest the involvement of Wnt and calcium signaling, vascular development and epigenetic mechanisms as potential mediators of the direct F1 generation actions of vinclozolin. (C) 2010 Elsevier Inc. All rights reserved.