Denitrification performance and biofilm characteristics using biodegradable polymers PCL as carriers and carbon source
Denitrification performance and biofilm characteristics using biodegradable polymers PCL as carriers and carbon source
复制标题
以可生物降解聚合物PCL为载体和碳源的反硝化性能及生物膜特性
DOI：
10.1016/j.chemosphere.2013.02.064
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发表时间：
2013-05-01
期刊：
影响因子：
8.8
通讯作者：
Wang, Jianlong
中科院分区：
文献类型：
--
作者：
Chu, Libing;Wang, Jianlong
采用生物聚合物聚己内酯(PCL)填充固定床生物反应器，通过反硝化去除地下水中的硝酸盐。PCL既是碳源，又是生物膜载体。在561d的长期运行中，考察了该反应器的脱氮性能、生物可降解载体上的生物膜特性以及固体碳源的降解和利用。在温度高于24℃的条件下，出水硝酸盐平均浓度低于3.7mgN·L-1，水力停留时间为3~6h时，总氮的去除可达95%以上，亚硝酸盐和氨氮保持在较低水平(分别低于0.32mgN·L-1和0.78mgN·L-1)。计算的PCL用量在1.6~3.7g PCL g(-1)NO3-N之间。用过的PCL的FT-IR谱图表明，PCL发生了水解链的断裂。生物膜的焦磷酸测序分析表明，黄杆菌属的生物膜占了大部分的序列。嗜氢菌属、未培养的红景天科细菌和硫化弧菌在PCL生物膜中高度丰富。微电极数据表明，生物膜的平均厚度在800g左右，反硝化活性最强的区域是300~500亩的生物膜，反硝化活性最高可达400 mU·mol·cm~(-3)·h~(-1)。(C)2013爱思唯尔有限公司。保留所有权利。
In this study, a fixed bed bioreactor filled with biopolymer polycaprolactone (PCL) was developed to remove nitrate from groundwater through denitrification. PCL serves both as carbon source and biofilm carriers. During a long-term operation of 561 d, the performance of the reactor in nitrogen removal, characteristics of biofilm attached to biodegradable carriers, and the hydrolysis and utilization of solid carbon source were studied. With temperatures exceeding 24 degrees C, the average nitrate concentration in the effluent was lower than 3.7 mg N L-1, and more than 95% of TN was removed at hydraulic retention time of 3-6 h. Nitrite and ammonium remained at low levels (less than 0.32 and 0.78 mg N L-1, respectively). The calculated PCL amount consumed ranged between 1.6 and 3.7 g PCL g(-1) NO3-N. The FT-IR spectrum of the used PCL indicated the chain scission by hydrolytic degradation. A pyrosequencing analysis of the biofilm showed that genus Diaphorobacter belonging to family Comamonadaceae accounted for most of the sequences. Bacteria of genera Hydrogenophaga, Rhodocyclaceae uncultured and Desulfovibrio were highly enriched in the PCL biofilm. Microelectrode data indicated that the biofilm had an average thickness of around 800 gm and the intensive denitrification activities occurred in the area of 300-500 mu m of biofilm with values up to 400 mu mol cm(-3) h(-1). (C) 2013 Elsevier Ltd. All rights reserved.