A permissive role of Notch in maintaining the DV affinity boundary of the Drosophila wing
A permissive role of Notch in maintaining the DV affinity boundary of the Drosophila wing
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DOI：
10.1016/j.ydbio.2008.07.028
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发表时间：
2008-10-01
影响因子：
2.7
通讯作者：
Milan, Marco
中科院分区：
文献类型：
--
作者：
Becam, Isabelle;Milan, Marco
细胞亲和性有助于将细胞组织成组织和器官。果蝇的四肢和脊椎动物的中枢神经系统被细分为不混合的相邻种群。这些细胞群被称为隔室。由Notch受体介导的细胞相互作用与隔室边界的规范有关。然而，Notch在这一过程中的贡献仍然存在争议。在过去的几年里，Notch的指导作用和该途径的转录要求受到了质疑。由于其在脊椎动物和无脊椎动物的发育边界中的核心作用，我们重新评估了Notch及其信号通路在维持果蝇翅膀背侧（D）和腹侧（V）隔间之间的亲和力差异方面的贡献。在这里，我们提出的证据表明，不受限制的，低水平的Notch足以形成一个稳定的DV亲和力边界。Notch蛋白的切割、胞内结构域的释放以及Notch通过无毛转录因子抑制子的转录功能在该过程中是必需的并且是足够的。我们的数据支持Notch在维持DV亲和力边界方面的容许作用。这与Notch在执行DV边界组织活动中的指导作用形成鲜明对比。(c)2008年爱思唯尔公司All rights reserved.
Cell affinities can contribute to organizing cells into tissues and organs. Drosophila limbs and the vertebrate central nervous system are subdivided into adjacent populations that do not mix. These cell populations are called compartments. Cell interactions mediated by receptor Notch have been implicated in the specification of compartment boundaries. However, the contribution of Notch in this process remains controversial. The instructive role of Notch and the transcriptional requirement of the pathway have been questioned in the last few years. Due to its central role in making developmental boundaries in vertebrates and invertebrates, we have reevaluated the contribution of Notch and its signaling pathway in the maintenance of an affinity difference between dorsal (D) and ventral (V) compartments in the Drosophila wing. Here we present evidence that unrestricted, low levels of Notch are sufficient for the formation of a stable DV affinity boundary. Cleavage of the Notch Protein, release of the intracellular domain and a transcriptional function of Notch via the Suppressor of Hairless transcription factor are required and sufficient in this process. Our data support a permissive role of Notch in maintaining the DV affinity boundary. This contrasts with the instructive role of Notch in executing the organizing activity of the DV boundary. (c) 2008 Elsevier Inc. All rights reserved.