Bone marrow stromal cells infused into the cerebrospinal fluid promote functional recovery of the injured rat spinal cord with reduced cavity formation
Bone marrow stromal cells infused into the cerebrospinal fluid promote functional recovery of the injured rat spinal cord with reduced cavity formation
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DOI：
10.1016/j.expneurol.2004.01.021
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发表时间：
2004-06-01
影响因子：
5.3
通讯作者：
Ide, C
中科院分区：
文献类型：
--
作者：
Ohta, M;Suzuki, Y;Ide, C
本实验观察了骨髓基质细胞（BMSCs）对大鼠脊髓损伤的修复作用及对行为学的改善作用。在T8-9节段采用重物撞击法造成脊髓挫伤，取同品系骨髓间充质干细胞经第四脑室注入脑脊液（CSF）。骨髓间充质干细胞经脑脊液转运至脊髓，大部分贴附于脊髓表面，少数侵入病变区，BBB评分高于对照组，脊髓腔体积小于对照组。移植的细胞数量逐渐减少，并在注射后3周从脊髓中消失。添加CSF的培养基（250穆尔，3毫升培养基）结果表明，BMSCs可通过向脑脊液中分泌营养因子或与宿主脊髓组织接触而发挥减少空洞和改善行为的作用在老鼠身上的作用考虑到BMSC可用于自体移植，并且移植物的CSF输注对患者造成的负担最小，本研究的结果对于BMSC在脊髓损伤治疗中的临床应用具有重要意义和前景。(C)2004年爱思唯尔公司All rights reserved.
The effects of bone marrow stromal cells (BMSCs) on the repair of injured spinal cord and on the behavioral improvement were studied in the rat. The spinal cord was injured by contusion using a weight-drop at the level of T8-9, and the BMSCs from the bone marrow of the same strain were infused into the cerebrospinal fluid (CSF) through the 4th ventricle. BMSCs were conveyed through the CSF to the spinal cord, where most BMSCs attached to the spinal surface although a few invaded the lesion.The BBB score was higher, and the cavity volume was smaller in the rats with transplantation than in the control rats. Transplanted cells gradually decreased in number and disappeared from the spinal cord 3 weeks after injection. The medium supplemented by CSF (250 mul in 3 ml medium) harvested from the rats in which BMSCs had been injected 2 days previously promoted the neurosphere cells to adhere to the culture dish and to spread into the periphery.These results suggest that BMSCs can exert effects by producing some trophic factors into the CSF or by contacting with host spinal tissues on the reduction of cavities and on the improvement of behavioral function in the rat. Considering that BMSCs can be used for autologous transplantation, and that the CSF infusion of transplants imposes a minimal burden on patients, the results of the present study are important and promising for the clinical use of BMSCs in spinal cord injury treatment. (C) 2004 Elsevier Inc. All rights reserved.