Deterministic Assembly of Complex Bacterial Communities in Guts of Germ-Free Cockroaches
Deterministic Assembly of Complex Bacterial Communities in Guts of Germ-Free Cockroaches
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DOI：
10.1128/aem.03700-15
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发表时间：
2016-02-01
影响因子：
4.4
通讯作者：
Brune, Andreas
中科院分区：
文献类型：
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作者：
Mikaelyan, Aram;Thompson, Claire L.;Brune, Andreas
白蚁肠道菌群在木质纤维素的共生消化过程中起着重要作用。然而，塑造微生物群落结构的因素仍然知之甚少。由于白蚁不能在无菌条件下饲养，我们建立了密切相关的蟑螂Shelfordella lateralis作为无菌模型来研究微生物群落组装和宿主-微生物相互作用。在这项研究中，我们确定了蟑螂接种白蚁和小鼠肠道菌群的细菌组合物的组成，使用焦磷酸测序分析其16 S rRNA基因。虽然外来接种物中存在的谱系的异生群落的组成的影响，其结构类似于传统的蟑螂。在传统蟑螂中丰富但在外来接种物中罕见的细菌分类群，如Dysgonomona和Parabacteroides spp.，在异生物群落中被选择性地富集。然而，供体特异性分类群，如限于白蚁肠道微生物群的内微生物或螺旋体谱系，要么无法殖民无菌蟑螂，要么只形成小种群。将异生物质蟑螂暴露于常规成年蟑螂中，恢复了它们正常的微生物群，这表明本土血统的竞争力超过了外来血统。我们的研究结果提供了实验证据，证明昆虫复杂肠道微生物群的组装是确定性的。
The gut microbiota of termites plays important roles in the symbiotic digestion of lignocellulose. However, the factors shaping the microbial community structure remain poorly understood. Because termites cannot be raised under axenic conditions, we established the closely related cockroach Shelfordella lateralis as a germ-free model to study microbial community assembly and host-microbe interactions. In this study, we determined the composition of the bacterial assemblages in cockroaches inoculated with the gut microbiota of termites and mice using pyrosequencing analysis of their 16S rRNA genes. Although the composition of the xenobiotic communities was influenced by the lineages present in the foreign inocula, their structure resembled that of conventional cockroaches. Bacterial taxa abundant in conventional cockroaches but rare in the foreign inocula, such as Dysgonomonas and Parabacteroides spp., were selectively enriched in the xenobiotic communities. Donor-specific taxa, such as endomicrobia or spirochete lineages restricted to the gut microbiota of termites, however, either were unable to colonize germ-free cockroaches or formed only small populations. The exposure of xenobiotic cockroaches to conventional adults restored their normal microbiota, which indicated that autochthonous lineages outcompete foreign ones. Our results provide experimental proof that the assembly of a complex gut microbiota in insects is deterministic.