Enhancement of Lymphatic Vessels in the Superficial Layer in a Rat Model of a Lymphedematous Response.
Enhancement of Lymphatic Vessels in the Superficial Layer in a Rat Model of a Lymphedematous Response.
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DOI：
10.1097/gox.0000000000001770
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Kimata Y
中科院分区：
文献类型：
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作者：
Matsumoto K;Kosaka J;Suami H;Kimata Y
淋巴水肿时淋巴管的形态和组织学行为尚未在实验动物中得到很好的分析。本研究旨在阐明大鼠急性淋巴水肿后淋巴管的再生过程。急性淋巴水肿是由大鼠后肢截肢和再植手术引起的。使用荧光淋巴造影术和染料注射追踪淋巴流量的恢复。用免疫组织化学方法检测和定量浅层和深层淋巴管的形态和数量。术后第3天肿胀最严重，浅层淋巴管数量明显增多且最多。术后第3天浅层也可见回流和溢流。术后第14天淋巴管数量有所减少，但仍明显高于对照组，肿胀程度降至对照组水平。而深层淋巴管数量有增加的趋势，但术后第3、7、14天差异无统计学意义。我们已经获得了可靠的证据，表明在诱导颞叶淋巴水肿后，浅层淋巴管和深层淋巴管的潜能存在差异。对动物模型中淋巴水肿反应的进一步分析可以为临床治疗淋巴水肿带来的挑战提供新的见解。
The morphologic and histologic behavior of lymphatic vessels in lymphedema has not been well analyzed using laboratory animals. The purpose of the present study was to elucidate the regeneration process of lymphatic vessels after acute lymphedema in a rat model. The acute lymphedema was induced by an amputation and a replantation surgery on a rat hind limb. Recovery of lymphatic flow was traced using fluorescent lymphography with dye injection. The morphology and number of lymphatic vessels were immunohistochemically detected and quantified in both superficial and deep layers. The swelling was the most severe, and the number of lymphatic vessels in the superficial layer was significantly and maximally increased on postoperative day 3. Backflows and overflows were also detectable in the superficial layer on postoperative day 3. The number of lymphatic vessels had decreased but remained significantly higher than that in the controls on postoperative day 14, when the swelling decreased to the levels in the controls. In contrast, the number of lymphatic vessels in the deep layer showed a tendency toward increased numbers; however, it was not statistically significant on postoperative day 3, 7, or 14. We have obtained solid evidence showing the differential potency of lymphatic vessels between the superficial and the deep layers after temporal lymphedematous induction. Further analysis of lymphedematous responses in animal models could provide new insights into the challenges associated with the clinical treatment of lymphedema.