Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia.
Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia.
复制标题
DOI：
10.1371/journal.pone.0177559
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kebede A
中科院分区：
文献类型：
--
作者：
Mohammed H;Kassa M;Assefa A;Tadesse M;Kebede A
在疟疾流行区，恶性疟原虫感染的特点是广泛的遗传多样性。描述这种多样性可以提供有关当地疟疾情况的重要信息。本研究使用高度多态性裂殖子表面蛋白2基因评估埃塞俄比亚西北部Pawe地区恶性疟原虫的遗传多样性程度。2013年9月至12月，从Pawe Health Centre就诊的无并发症恶性疟原虫患者中共收集了92株分离株。使用Chelex方法提取基因组DNA，并在来自靶向FC 27和3D 7/IC的等位基因家族的msp 2的巢式PCR（框3）的DNA产物的凝胶电泳之后通过长度多态性进行分析。有22个不同的msp 2等位基因，11个对应于3D 7/ IC，11个对应于FC 27等位基因家族。msp 2 3D 7/IC等位基因家族的分离株频率（51%）高于FC 27（49%）。大多数分离株（76%）含有多重感染，总体平均多重感染数为2.8（95%CI 2.55-3.03）。msp 2的杂合性指数为0.66。年龄或寄生虫密度的感染复数无统计学显著差异。本研究结果表明，恶性疟原虫多态性在埃塞俄比亚西北部广泛存在，大多数感染由多个克隆组成。
In malaria endemic regions, Plasmodium falciparum infection is characterized by extensive genetic diversity. Describing this diversity provides important information about the local malaria situation. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Pawe district, North West Ethiopia, using the highly polymorphic merozoite surface protein 2 gene. Atotal of 92 isolates from patients with uncomplicated P. falciparum attending Pawe Health Centre were collected from September to December 2013. Genomic DNA was extracted using the Chelex method and analyzed by length polymorphism following gel electrophoresis of DNA products from nested PCR of msp2 (block 3), targeting allelic families of FC27 and 3D7/IC. There were twenty-two different msp2 alleles, 11 corresponding to the 3D7/ IC and 11 to the FC27 allelic family. The frequency of isolates of the msp2 3D7/IC allelic familywas higher (51%) compared to FC27 (49%). The majority of the isolates (76%) contained multiple infections andthe overall mean multiplicity of infection was 2.8 (CI 95% 2.55–3.03). The heterozygosity index was 0.66 for msp2. There was no statically significant difference in the multiplicity of infection by age or parasite density. The results of this study show that P.falciparum polymorphismsare extensive in Northwest Ethiopia and most of the infections are composed of multiple clones.