INCREASE OF CHALCONE SYNTHASE MESSENGER-RNA IN PATHOGEN-INOCULATED SOYBEANS WITH RACE-SPECIFIC RESISTANCE IS DIFFERENT IN LEAVES AND ROOTS
INCREASE OF CHALCONE SYNTHASE MESSENGER-RNA IN PATHOGEN-INOCULATED SOYBEANS WITH RACE-SPECIFIC RESISTANCE IS DIFFERENT IN LEAVES AND ROOTS
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DOI：
10.1104/pp.91.3.911
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发表时间：
1989-11-01
期刊：
影响因子：
7.4
通讯作者：
KUHN, DN
中科院分区：
文献类型：
--
作者：
DHAWALE, S;SOUCIET, G;KUHN, DN
大豆(甘氨酸max [L.][Merr.])通过产生类异黄酮衍生的植物抗毒素来应对病原体。查尔酮合成酶（Chalcone synthase， CHS）是类黄酮/异黄酮生物合成途径的第一酶。我们研究了接种两种不同种特异性相互作用(叶片与丁香假单胞菌（Psg）和根与真菌巨藻疫霉（Phytophthora megasperma f. sp. glycinea, Pmg）后，CHS mRNA和其他特异性mRNA的稳态水平随接种时间的增加而变化。接种Psg无毒小种后，大豆叶片CHS mRNA的表达量显著增加，而接种Psg有毒小种或水后，CHS mRNA的表达量无显著增加。相比之下，在接种了无毒或有毒Pmg种的游动孢子的根中，CHS mRNA的增加是相似的。接种了病原菌的根中CHS mRNA显著升高，而未接种的对照中CHS mRNA无显著升高。富羟基脯氨酸糖蛋白（HRGP）在植物受伤或接种病原体时均有升高的趋势。我们在这里报道，在接种了无毒Pmg小种的根中，HRGP mRNA水平高于接种了有毒小种的根，但接种两种小种都导致HRGP mRNA水平显著高于对照。钙调素和泛素mRNA在未接种或接种的根和叶中均未增加。本文还讨论了大豆在不同器官中表达不同抗性的可能性。
Soybeans (Glycine max [L.] Merr.) respond to pathogens by producing isoflavonoid-derived phytoalexins. Chalcone synthase (CHS) is the first enzyme of the flavonoid/isoflavonoid biosynthetic pathway. We investigated changes in the steady state levels of CHS mRNA andother specific mRNAs at increasing times after inoculation in two different race-specific interactions, one between leaves and the bacterium Pseudomonas syringae pv glycinea (Psg), and one between roots and the fungus, Phytophthora megasperma f. sp. glycinea (Pmg). The amount of CHS mRNA increases significantly in soybean leaves inoculated with an avirulent race of Psg but not with a virulent race or water. In contrast, the increase in CHS mRNA is similar in roots inoculated with zoospores of either an avirulent or virulent race of Pmg. CHS mRNA increases significantly in pathogen inoculated roots but not in uninoculated controls. Hydroxyproline-rich glycoprotein (HRGP) has been observed by others to increase in wounded or pathogen-inoculated plants. We report here that HRGP mRNA levels are greater in roots inoculated with an avirulent Pmg race than with a virulent race, but inoculation with either race causes a significant increase in HRGP mRNA with respect to controls. Calmodulin or ubiquitin mRNA do not increase in either uninoculated or inoculated roots and leaves. The possibility that race-specific resistance in soybeans is expressed differently in different organs of the plant is discussed.