Non-toxic retention of nanoceria in murine eyes
Non-toxic retention of nanoceria in murine eyes
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DOI：
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发表时间：
2016-10
期刊：
影响因子：
2.2
通讯作者：
Xue Cai;S. Seal;J. McGinnis
中科院分区：
文献类型：
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作者：
Xue Cai;S. Seal;J. McGinnis
目的研究表明，纳米CeO_3具有独特的特性和催化活性，一次玻璃体内注射后可在视网膜内保留1年以上，可用于多种眼科疾病的治疗。这项研究的目的是确定纳米氧化钙在眼睛中的滞留是否会导致炎症或不良副作用。方法用不同浓度的纳米钙玻璃体腔内注射于胎龄30天的野生型(C57BL/6J)小鼠。通过分析光感受器特异基因的表达以及视网膜的结构和功能来评估光感受器的健康状况。通过分析血管系统、炎性细胞因子的表达和眼内细胞的浸润来研究纳米氧化钙的作用。结果单次玻璃体内注射纳米氧化钙后，视网膜结构、功能及细胞因子基因表达均未见改变。结论纳米氧化钙的剂量为17.2~1720 ng/只眼，注射后30d内不会对视网膜结构和功能造成任何损害。眼内未见细胞浸润，炎性反应也未见增加。我们的数据表明，纳米氧化钙用于治疗各种眼病是安全的。
Purpose We have shown that cerium oxide nanoparticles (nanoceria), with unique characteristics and catalytic activities, are retained in the retina for more than 1 year after a single intravitreal injection and can be potentially used for the treatment of a variety of eye diseases. The objective of this study is to determine whether the retention of nanoceria in the eye causes inflammation or adverse side effects. Methods Wild-type (C57BL/6J) mice at P30 were intravitreally injected with several concentrations of nanoceria. The health of the photoreceptors was assessed by analyzing the expression of photoreceptor-specific genes, and the retinal structure and function. The effect of nanoceria was investigated by analyzing of the vascular system, the expression of inflammatory cytokines, and cellular infiltration into the eye. Results Our data showed that there were no changes in the retinal structure or function, or cytokine gene expression following a single intravitreal injection of nanoceria. Conclusions Nanoceria, at doses ranging from 17.2 ng to 1720 ng per eye, do not cause any damage to the retinal structure and function by 30 days post injection. No cellular infiltration and no increases in inflammatory responses were found in the eyes. Our data indicate that nanoceria are safe to use for treatment of a variety of eye diseases.