Multiplexed, rapid point of care platform to quantify allergen-specific IgE.
Multiplexed, rapid point of care platform to quantify allergen-specific IgE.
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DOI：
10.1109/iembs.2011.6090069
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ünlü MS
中科院分区：
文献类型：
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作者：
Monroe MR;Reddington AP;Cretich M;Chiari M;Little F;Ünlü MS
探针固定在微阵列上的变化阻碍了在医疗环境中做出高质量、自信和统计相关结论的能力。为了解决这个问题，我们已经开发了一种校准的、紧凑的、廉价的、多路复用的、双模态的床旁检测平台，其校准和关联无标记测量的表面探针密度与捕获的标记的二抗，独立于芯片到芯片的可变性，并且改进了现有的诊断技术。我们已经确定了我们提出的平台的四个主要技术优势：基于用于检测的荧光团进行单点分析的能力，由于优化的底物而导致的荧光信号的10倍增益，使用组合的荧光和无标记模式来精确测量各种系统的探针和结合靶的密度的校准的定量方法，紧凑的测量平台可在医生办公室提供可靠和快速的结果。我们已经用公式表示了与表面结合的探针的量与IgG、β-乳球蛋白、Ara h 1花生过敏原和Phl 5a蒂莫西草过敏原的捕获靶标的所得荧光之间超过90%的线性相关性。
Variation of probe immobilization on microarrays hinders the ability to make high quality, assertive and statistically relevant conclusions needed in the healthcare setting. To address this problem, we have developed a calibrated, compact, inexpensive, multiplexed, dual modality point-of-care detection platform that calibrates and correlates surface probe density measured label-free to captured labeled secondary antibody, is independent of chip-to-chip variability, and improves upon existing diagnostic technology. We have identified four major technological advantages of our proposed platform: the capability to perform single spot analysis based on the fluorophore used for detection, a 10-fold gain in fluorescence signal due to optimized substrate, a calibrated, quantitative method that uses the combined fluorescent and label-free modalities to accurately measure the density of probe and bound target for a variety of systems, and a compact measurement platform offering reliable and rapid results at the doctor’s office. Already, we have formulated over a 90% linear correlation between the amount of probe bound to surface and the resulting fluorescence of captured target for IgG, β-lactoglobulin, Ara h 1 peanut allergen, and Phl 5a Timothy grass allergen.