Detection of a Foreign Protein in Milk Using Surface-Enhanced Raman Spectroscopy Coupled with Antibody-Modified Silver Dendrites
Detection of a Foreign Protein in Milk Using Surface-Enhanced Raman Spectroscopy Coupled with Antibody-Modified Silver Dendrites
复制标题
DOI：
10.1021/ac1032353
复制
发表时间：
2011-03-01
影响因子：
7.4
通讯作者：
Labuza, Theodore P.
中科院分区：
文献类型：
--
作者：
He, Lili;Rodda, Tom;Labuza, Theodore P.
在此，我们开发了一种快速，简单的方法，使用表面增强拉曼光谱（Sers）结合抗体修饰的银树枝状分子检测卵清蛋白（OVA），卵白色蛋白，引入到全脂牛奶。首先通过使用抗体修饰的银枝晶从牛奶中捕获OVA，然后通过拉曼光谱在银枝晶上直接测量。结果表明，基于主成分分析，该方法能够在30 min内检测磷酸盐缓冲盐水（PBS）中0.1 μ g/mL和牛奶中5 μ g/mL的OVA。该方法具有广泛的应用前景，如过敏性蛋白质检测和复杂基质中的生物恐怖剂检测。
Herein we developed a rapid and simple method which used surface-enhanced Raman spectroscopy (SERS) coupled with antibody-modified silver dendrites to detect ovalbumin (OVA), the egg white protein, introduced into whole milk. OVA was first captured out of milk by use of antibody-modified silver dendrites and then directly measured on the silver dendrites by Raman spectroscopy. Results show that this method is capable of detecting OVA at 0.1 mu g/mL in phosphate buffered saline (PBS) and 5 mu g/mL in milk within 30 min based on the principal component analysis. This method has the potential for wide use in areas such as allergenic protein detection and bioterrorism agent detection in complex matrixes.