Investigation of feather follicle morphogenesis and the expression of the Wnt/β-catenin signaling pathway in yellow-feathered broiler chick embryos
Investigation of feather follicle morphogenesis and the expression of the Wnt/β-catenin signaling pathway in yellow-feathered broiler chick embryos
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DOI：
10.1080/00071668.2020.1758302
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发表时间：
2020-04
影响因子：
2
通讯作者：
W. Xie;M. J. Chen;Shi-guang Jiang;Hui-chao Yan;X. Q. Wang;C. Gao
中科院分区：
文献类型：
--
作者：
W. Xie;M. J. Chen;Shi-guang Jiang;Hui-chao Yan;X. Q. Wang;C. Gao
摘要1.采用苏木精-伊红（H&E）染色法和Western blot法研究了黄羽肉鸡胚胎羽毛发育过程中羽囊形态发生的模式和Wnt/β-catenin信号通路在皮肤中的表达。2.结果表明：胚胎期（E7 ~ E9），皮肤突起;胚胎期（E10 ~ E11），羽芽伸长，前后不对称，近远不对称;胚胎期（E12），表皮内陷，形成初级羽囊。E13时，羽芽基部的表皮和羽囊的形成进一步内陷到真皮中。到E15，Pf的形成基本完成，并出现次级羽囊（Sfs）。推测Pfs和Sfs是独立发展的，Pfs的发生早于Sfs。3. Pf密度的定量测量在E15达到最大值，然后逐渐下降。Sf密度从E15开始增加。4.β-catenin、TCF 4、cyclin D1和c-Myc蛋白表达水平在E8-E12期间显著升高（P < 0.05），从E13到孵化日（DOH）逐渐降低（P < 0.05）。β-连环蛋白免疫定位信号强度测定的结果与Western印迹测定的结果一致。5.总的来说，结果表明Wnt/β-catenin信号通路对于促进羽囊的发育是必不可少的，特别是在E7-E15期间。
ABSTRACT 1. This study investigated the pattern of feather follicle morphogenesis and the expression of the Wnt/β-catenin signalling pathway in the skin of yellow-feathered broiler chick embryos during feather development, using haematoxylin and eosin (H&E) staining and Western blot assays, respectively. 2. The results showed that the skin displayed protrusions during embryonic days E7-E9, feather buds elongated during E10-E11 with anterior-posterior and proximal-distal asymmetries, and the epidermis invaginated to form the primary feather follicles (Pfs) at E12. At E13, the formation of the feather follicle and the epidermis at the base of the feather bud further invaginated into the dermis. By E15, Pf formation was essentially complete, and secondary feather follicles (Sfs) appeared. It was speculated that Pfs and Sfs developed independently and that Pfs occurred earlier than Sfs. 3. Quantitative measurements of Pf density reached a maximum at E15 and then decreased gradually. Sf density started to increase from E15. 4. Protein expression levels of β-catenin, TCF4, cyclin D1, and c-Myc were significantly increased during E8-E12 (P < 0.05) and then decreased from E13 to the day of hatching (DOH) (P < 0.05). The result of the β-catenin immunolocalisation signal intensity assay was consistent with the result of the Western blot assay. 5. Collectively, the results indicated that the Wnt/β-catenin signalling pathway is essential for promoting the development of feather follicles, especially during E7-E15.