Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures.
Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures.
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DOI：
10.1371/journal.pone.0176031
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ro S
中科院分区：
文献类型：
--
作者：
Lee MY;Ha SE;Park C;Park PJ;Fuchs R;Wei L;Jorgensen BG;Redelman D;Ward SM;Sanders KM;Ro S
转录组尺度的数据可以揭示理解特定细胞功能和生物过程背后的潜在分子机制的基本线索。转录组学是一个不断发展的研究领域，用于生物标志物的发现。Cajal （ICC）间质细胞作为胃肠道（GI）平滑肌的慢波电起搏器，其转录组学特征尚未被发现。利用copgfp标记的ICC小鼠和流式细胞术，我们从小鼠小肠和结肠中分离出ICC群体，并获得了它们的转录组。在分析转录组时，我们确定了一组独特的icc限制性标记，包括转录因子、表观遗传酶/调节剂、生长因子、受体、蛋白激酶/磷酸酶和离子通道/转运体。这一分析为ICC在GI生理学中的细胞和生物学功能提供了新的和独特的见解。此外，我们基于UCSC基因组数据库构建了交互式ICC基因组浏览器（http://med.unr.edu/physio/transcriptome）。据我们所知，这是第一个在线资源，提供了一个全面的库，所有已知的基因转录本表达在初级ICC。我们的基因组浏览器为研究ICC中基因的替代表达提供了新的视角，并为未来的功能研究提供了有价值的参考。
Transcriptome-scale data can reveal essential clues into understanding the underlying molecular mechanisms behind specific cellular functions and biological processes. Transcriptomics is a continually growing field of research utilized in biomarker discovery. The transcriptomic profile of interstitial cells of Cajal (ICC), which serve as slow-wave electrical pacemakers for gastrointestinal (GI) smooth muscle, has yet to be uncovered. Using copGFP-labeled ICC mice and flow cytometry, we isolated ICC populations from the murine small intestine and colon and obtained their transcriptomes. In analyzing the transcriptome, we identified a unique set of ICC-restricted markers including transcription factors, epigenetic enzymes/regulators, growth factors, receptors, protein kinases/phosphatases, and ion channels/transporters. This analysis provides new and unique insights into the cellular and biological functions of ICC in GI physiology. Additionally, we constructed an interactive ICC genome browser (http://med.unr.edu/physio/transcriptome) based on the UCSC genome database. To our knowledge, this is the first online resource that provides a comprehensive library of all known genetic transcripts expressed in primary ICC. Our genome browser offers a new perspective into the alternative expression of genes in ICC and provides a valuable reference for future functional studies.