[Multilineage differentiation of human dental pulp cells and periodontal ligament cells in vitro].
[Multilineage differentiation of human dental pulp cells and periodontal ligament cells in vitro].
复制标题
人牙髓细胞和牙周膜细胞的体外多向分化[J].
DOI：
10.3321/j.issn:1002-0098.2008.08.013
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Lu Liu
中科院分区：
文献类型：
--
作者：
Xi Wei;Li;J. Ling;Lu Liu
目的
目的比较人牙髓细胞（DPC）和牙周膜细胞（PDLC）在体外的多向分化潜能，并鉴定其干细胞特性。
方法
通过酶消化分离人DPC和PDLC。通过流式细胞术研究STRO-1表达。诱导细胞向成牙/成骨分化、成脂分化和成软骨分化。采用vonKossa染色、抗骨钙素（OCN）和抗牙本质涎蛋白（DSP）免疫细胞化学、油红O染色、阿尔新蓝染色、抗II型胶原免疫细胞化学和真实的实时RT-PCR检测DPC和PDLC的多向分化能力。
结果
在DPC和PDLC中观察到集落形成，STRO-1阳性率分别为（16.5% ± 4.2%）和（11.6% ± 1.1%）。与83.3%的PDLC样品相比，在100%的DPC样品中证明了多系分化。诱导后DPC和PDLC中OCN、牙本质涎磷蛋白（DSPP）、过氧化物酶体增殖物激活受体γ 2（PPARgamma 2）、脂蛋白脂酶（LPL）和II型胶原mRNA水平均显著上调（P < 0.001）。DPC与PDLC中OCN和PPARgamma 2 mRNA表达上调的比例有显著性差异（P < 0.001）。
结论
DPC和PDLC含有相似比例的间充质干细胞，并具有相当的多向分化能力。
OBJECTIVE
To compare the multilineage differentiation potential of human dental pulp cells (DPC) and periodontal ligament cells (PDLC) in vitro, and to identify the stem cell characteristics.
METHODS
Human DPC and PDLC were isolated by enzymatic digestion. STRO-1 expression was investigated by flow cytometry. Cells were induced to odontogenic/osteogenic differentiation, adipogenic differentiation and chondrogenic differentiation. The multilineage differentiation capacities of DPC and PDLC were evaluated by von Kossa stain, anti-osteocalcin (OCN) and anti-dentin sialoprotein (DSP) immunocytochemistry, oil red O stain, Alcian blue stain, anti-collagen type II immunocytochemistry, and real time RT-PCR.
RESULTS
Colony formation was observed in DPC and PDLC, with STRO-1 positive rate of (16.5% +/- 4.2%) and (11.6% +/- 1.1%) respectively. Multilineage differentiation was demonstrated in 100% of DPC samples in contrast to 83.3% of PDLC samples. OCN, dentin sialophosphoprotein (DSPP), peroxisome-proliferator-activated receptor gamma 2 (PPARgamma2), lipoprotein lipase (LPL) and collagen type II mRNA levels in DPC and PDLC were significantly upregulated after induction (P < 0.001). There were significant differences in the ratio of upregulation of OCN and PPARgamma2 mRNAs between DPC and PDLC (P < 0.001).
CONCLUSIONS
DPC and PDLC contain similar proportion of mesenchymal stem cells and possess comparable multilineage differentiation capacities.