Aptamers as affinity ligands for downstream processing
Aptamers as affinity ligands for downstream processing
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DOI：
10.1002/elsc.201100197
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发表时间：
2012-10
影响因子：
2.7
通讯作者：
J. Walter;F. Stahl;T. Scheper
中科院分区：
文献类型：
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作者：
J. Walter;F. Stahl;T. Scheper
适配子是一种单链人工合成的寡核苷酸，能够以高亲和力和特异性捕获其靶分子。因此，它们可以被认为是基于核酸的抗体的替代品，抗体与基于氨基酸的抗体相比有几个优势。因此，适配子可用于基于分子识别的不同应用，包括亲和分离。这篇综述将总结基于适体的亲和分离的最新进展；将讨论当前的局限性，并将突出可能的未来前景。第一部分将指出适配子在下游加工中的优势。这里，将从用户的角度讨论适配子的性质以及它们对下游处理的影响。第二部分是文献综述，重点介绍了基于适体的蛋白质分离方法。最后，将说明适体的一些缺点，并提出克服这些限制的可能性。在适配子选择和合成领域的新技术有望进一步促进在下游加工中将适配子用作亲和配体。
Aptamers are single‐stranded synthetic oligonucleotides that are able to capture their target molecule with high affinity and specificity. Therefore, they can be thought of as nucleic acid‐based alternative to antibodies, which have several advantages over their amino acid‐based counterparts. Consequently, aptamers can be used in different applications based on molecular recognition including affinity separations. This review will summarize the state‐of‐the‐art in aptamer‐based affinity separations; will discuss the current limitations and will highlight possible future prospects. The first part will point out the advantages of aptamers in downstream processing. Here, the properties of aptamers will be discussed along with their implications on downstream processing from a user's point of view. In the second part, a brief summary of the literature is given with focus on aptamer‐based separation of proteins. Finally, some drawbacks of aptamers will be illustrated and possibilities to overcome these limitations will be suggested. New technologies in the fields of aptamer selection and synthesis are expected to further promote the use of aptamers as affinity ligands in downstream processing.