The vimentin-tubulin binding site peptide (Vim-TBS.58-81) crosses the plasma membrane and enters the nuclei of human glioma cells
The vimentin-tubulin binding site peptide (Vim-TBS.58-81) crosses the plasma membrane and enters the nuclei of human glioma cells
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DOI：
10.1016/j.ijpharm.2011.04.067
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发表时间：
2012-02-14
影响因子：
5.8
通讯作者：
Eyer, Joel
中科院分区：
文献类型：
--
作者：
Balzeau, Julien;Peterson, Alan;Eyer, Joel
细胞穿透肽（Cell-penetrating peptides，CPP）是一种具有跨膜转运能力的多肽类、蛋白质类、核酸类及其他生物活性物质。在这里，我们描述了一种源自中间丝蛋白波形蛋白的新型细胞穿透肽的特征，称为Vim-TBS。58-81。我们发现，它通过内吞作用从胶质母细胞瘤细胞系进入细胞，分布在整个细胞质和细胞核中。此外，当偶联到促增殖肽P10时，它定位于细胞核，抑制细胞增殖。因此，Vim-TBS.58-81肽代表了用于将肽和潜在的宽范围的货物递送到细胞核的有效载体。(C)2011 Elsevier B. V.保留所有权利。
Cell-penetrating peptides (CPPs) can translocate through the plasma membrane and localize in different cell compartments providing a promising delivery system for peptides, proteins, nucleic acids, and other products. Here we describe features of a novel cell-penetrating peptide derived from the intermediate filament protein vimentin, called Vim-TBS.58-81. We show that it enters cells from a glioblastoma line via endocytosis where it distributes throughout the cytoplasm and nucleus. Moreover, when coupled to the pro-apoptogenic peptide P10, it localizes to the nucleus inhibiting cell proliferation. Thus, the Vim-TBS.58-81 peptide represents an effective vector for delivery of peptides and potentially a broad range of cargos to the nucleus. (C) 2011 Elsevier B.V. All rights reserved.