Global spatial analysis of Arabidopsis natural variants implicates 5'UTR splicing of LATE ELONGATED HYPOCOTYL in responses to temperature.
Global spatial analysis of Arabidopsis natural variants implicates 5'UTR splicing of LATE ELONGATED HYPOCOTYL in responses to temperature.
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DOI：
10.1111/pce.13188
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Nimmo HG
中科院分区：
文献类型：
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作者：
James AB;Sullivan S;Nimmo HG
植物如何感知和响应温度一直是植物科学中的一个重要问题。温度感知和信号转导可能通过初级mRNA转录物的温度敏感性分子内折叠发生。最近的研究表明，时钟元件LHY的5′UTR中的第一内含子的保留在响应温度变化中起作用。在这里，我们确定了LHY 5′UTR中的一组单倍型，检查了它们的全球空间分布，并获得了单倍型可以影响LHY转录本温度依赖性剪接的证据。单倍型空间分布与全球生物气候变量和海拔的相关性与年平均温度和温度波动有关。相对罕见的孑遗类型加入与较低的平均温度和更大的温度波动和其他单倍型的空间分布可能是信息的进化过程中驱动殖民的生态系统。我们提出，单倍型可能具有不同的5′UTR前mRNA折叠热力学和/或基于反式作用RNA剪接因子结合的特定生物稳定性，其结果是中心时钟组件的可扩展剪接灵敏度，可能与特定的温度环境相适应。植物如何感知和响应温度一直是植物科学中的一个重要问题。我们研究了核心昼夜节律钟组成部分LHY（晚伸长下胚轴）5′UTR区域单倍型的全球空间分布，并获得了单倍型影响LHY转录本温度依赖性剪接的证据。我们的工作表明，可扩展的剪接灵敏度调整生物钟，以特定的温度环境。
How plants perceive and respond to temperature remains an important question in the plant sciences. Temperature perception and signal transduction may occur through temperature‐sensitive intramolecular folding of primary mRNA transcripts. Recent studies suggested a role for retention of the first intron in the 5′UTR of the clock component LATE ELONGATED HYPOCOTYL (LHY) in response to changes in temperature. Here, we identified a set of haplotypes in the LHY 5′UTR, examined their global spatial distribution, and obtained evidence that haplotype can affect temperature‐dependent splicing of LHY transcripts. Correlations of haplotype spatial distributions with global bioclimatic variables and altitude point to associations with annual mean temperature and temperature fluctuation. Relatively rare relict type accessions correlate with lower mean temperature and greater temperature fluctuation and the spatial distribution of other haplotypes may be informative of evolutionary processes driving colonization of ecosystems. We propose that haplotypes may possess distinct 5′UTR pre‐mRNA folding thermodynamics and/or specific biological stabilities based around the binding of trans‐acting RNA splicing factors, a consequence of which is scalable splicing sensitivity of a central clock component that is likely tuned to specific temperature environments. How plants perceive and respond to temperature remains an important question in the plant sciences. We investigated the global spatial distribution of haplotypes for the 5′UTR region of the core circadian clock component LATE ELONGATED HYPOCOTYL (LHY) and obtained evidence that haplotype influences temperature‐dependent splicing of LHY transcripts. Our work suggests that scalable splicing sensitivity tunes the circadian clock to specific temperature environments.