TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes.
TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes.
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DOI：
10.1093/mp/sst152
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发表时间：
2014-04
期刊：
影响因子：
27.5
通讯作者：
Danlin Zhu;Zhe Wu;Guangyun Cao;Jigang Li;Jia Wei;T. Tsuge;H. Gu;T. Aoyama;Li-jia Qu
中科院分区：
文献类型：
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作者：
Danlin Zhu;Zhe Wu;Guangyun Cao;Jigang Li;Jia Wei;T. Tsuge;H. Gu;T. Aoyama;Li-jia Qu
水是几乎所有生物体中最丰富的分子。水通道蛋白是在控制细胞含水量方面起关键作用的通道蛋白。在这里，我们报告了一个AP 2/EREBP家族转录因子在拟南芥，TRANSLUCENT绿色（TG），其在转基因植物中的过表达增强了耐旱性和玻璃化叶片的鉴定。TG蛋白定位于细胞核中，在体外结合DRE和GCC元件，并在酵母细胞中充当转录激活因子。基因芯片分析共发现了330个受TG调控的基因，其中5个基因编码水通道蛋白。瞬时表达实验表明，TG可直接与AtTIP 1;1、AtTIP 2;3和AtPIP 2;2等3个水通道蛋白基因的启动子结合，表明TG可直接调控这些基因的表达。此外，AtTIP 1;1的过表达导致转基因拟南芥植物中的玻璃化表型，类似于在TG过表达株系中观察到的表型。水注入到野生型叶重演玻璃化叶表型，这是逆转切断供水维管束。综上所述，我们的数据支持TG通过直接激活水通道蛋白基因的表达来控制拟南芥的水分平衡。
Water is the most abundant molecule in almost all living organisms. Aquaporins are channel proteins that play critical roles in controlling the water content of cells. Here, we report the identification of an AP2/EREBP family transcription factor in Arabidopsis thaliana, TRANSLUCENT GREEN (TG), whose overexpression in transgenic plants gave enhanced drought tolerance and vitrified leaves. TG protein is localized in the nucleus, binds DRE and GCC elements in vitro, and acts as a transcriptional activator in yeast cells. Microarray analysis revealed a total of 330 genes regulated by TG, among which five genes encode aquaporins. A transient expression assay showed that TG directly binds to the promoters of three aquaporin genes, such as AtTIP1;1, AtTIP2;3, and AtPIP2;2, indicating that TG directly regulates the expression of these genes. Moreover, overexpression of AtTIP1;1 resulted in vitrified phenotypes in transgenic Arabidopsis plants, similar to those observed in TG overexpression lines. Water injection into wild-type leaves recapitulated the vitrified leaf phenotypes, which was reversed by cutting off the water supply from vascular bundles. Taken together, our data support that TG controls water balance in Arabidopsis through directly activating the expression of aquaporin genes.