Two different cell-cycle processes determine the timing of cell division in Escherichia coli.
Two different cell-cycle processes determine the timing of cell division in Escherichia coli.
复制标题
两种不同的细胞周期过程决定了大肠杆菌细胞分裂的时间。
DOI：
10.7554/elife.67495
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发表时间：
2021-10-06
期刊：
影响因子：
7.7
通讯作者：
van Teeffelen S
中科院分区：
文献类型：
--
作者：
Colin A;Micali G;Faure L;Cosentino Lagomarsino M;van Teeffelen S
细胞必须控制细胞周期，以确保完成关键过程。在大肠杆菌中，细胞分裂是否与染色体复制或与复制无关的分裂间过程有关是有争议的。最近的一个模型表明，这两个过程可能会限制细胞分裂，在单细胞中的几率相当。在这里，我们通过监测缓慢生长的多代单细胞分裂和复制来实验测试这种可能性。然后我们扰动细胞宽度，导致复制终止和分裂之间的时间增加。因此，复制变得越来越限制细胞分裂，而出生和分裂之间的相关性和随后的复制启动事件之间保持。我们的实验支持这一假设，即染色体复制和复制独立的分裂过程可以限制细胞分裂：这两个过程在非扰动细胞中具有平衡的贡献，而我们的宽度扰动增加了复制独立过程限制的可能性。
Cells must control the cell cycle to ensure that key processes are brought to completion. In Escherichia coli, it is controversial whether cell division is tied to chromosome replication or to a replication-independent inter-division process. A recent model suggests instead that both processes may limit cell division with comparable odds in single cells. Here, we tested this possibility experimentally by monitoring single-cell division and replication over multiple generations at slow growth. We then perturbed cell width, causing an increase of the time between replication termination and division. As a consequence, replication became decreasingly limiting for cell division, while correlations between birth and division and between subsequent replication-initiation events were maintained. Our experiments support the hypothesis that both chromosome replication and a replication-independent inter-division process can limit cell division: the two processes have balanced contributions in non-perturbed cells, while our width perturbations increase the odds of the replication-independent process being limiting.