Immunohistochemical detection of meconium in the fetal lung: report of autopsied cases.
Immunohistochemical detection of meconium in the fetal lung: report of autopsied cases.
复制标题
胎肺胎便的免疫组织化学检测：尸检病例报告。
DOI：
10.1007/s00404-013-2862-4
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kanayama N et al.
中科院分区：
文献类型：
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作者：
Furuta N;Kanayama N et al.
胎粪是一种在妊娠中期之前很久就存在于胎儿小肠中的胆汁染色物质，随着肠壁的收缩而在肠腔中移动[1]。尽管胎粪吸入综合征可能会使新生儿死亡率和发病率恶化，但胎粪的病理生理学参与仍然存在争议[2]，因为尸检研究表明宫内感染和/或慢性缺氧的产前起源[2-4]。胎粪染色缺乏统一的诊断标准是造成混乱的原因之一。我们先前报道了锌粪卟啉I（ZnCP-I）在人胎粪中的特异性存在[5]，最近开发了ZnCP-I的特异性单克隆抗体[6]，并成功地将其应用于人胎盘、胎膜和脐带中胎粪的免疫染色[6]。在本研究中，我们将抗锌磷蛋白抗体1例对滨松大学1例早期新生儿死亡和8例妊娠28 ~ 38周宫内胎儿死亡后尸检肺进行免疫组化检查1996年至2003年在医院工作。石蜡块在10%甲醛中固定后，切成3 μm的切片。将小鼠单克隆抗ZnCP-I抗体（120 ng/ml）或抗CD 68抗体（从初始液体稀释4倍; Dako Japan，Tokyo，Japan）应用于切片（1小时，室温）后，用聚合物检测试剂盒（ChemMate EnVisionTM; Dako Japan，Tokyo，Japan）进行检测，
Meconium is a bile-stained material present in the small bowel of fetuses long before mid-gestation, which moves in the intestinal lumen with contractions of the intestinal wall [1]. Although meconium aspiration syndrome could deteriorate neonatal mortality and morbidity, the pathophysiological involvement of meconium is still contentious [2], because autopsy studies suggested prenatal origins of intrauterine infection and/or chronic hypoxia [2–4]. The lack of universal diagnostic criteria for meconium staining is one of the reasons for the confusion. We previously reported the specific presence of zinc coproporphyrin I (ZnCP-I) in human meconium [5], recently developed a specific monoclonal antibody for ZnCP-I [6], and successfully applied it to the immunostaining of meconium in the human placenta, fetal membrane, and umbilical cord [6].In the present study, we applied the antibody for ZnCP-1 to immunohistochemical examination of the autopsied lung following one early neonatal death and eight intrauterine fetal deaths between 28 and 38 weeks of gestation at Hamamatsu University Hospital between 1996 and 2003. Paraffin blocks, after fixation in 10% formaldehyde, were cut into 3 μm sections. After the application of a mouse monoclonal anti-ZnCP-I antibody (120 ng/ml) or anti-CD 68 antibody (fourfold dilution from initial liquid; Dako Japan, Tokyo, Japan) to the sections (1 h, room temperature), detection was performed with a polymer detection kit (ChemMate EnVisionTM; Dako Japan, Tokyo, Japan),