SITES OF TRANSCRIPTION OF THE MULLERIAN-INHIBITING SUBSTANCE GENE IN MOUSE TESTIS
SITES OF TRANSCRIPTION OF THE MULLERIAN-INHIBITING SUBSTANCE GENE IN MOUSE TESTIS
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DOI：
10.1002/mrd.1080350209
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发表时间：
1993-06-01
影响因子：
2.5
通讯作者：
STALVEY, JRD
中科院分区：
文献类型：
--
作者：
AO, A;ERICKSON, RP;STALVEY, JRD
应用逆转录聚合酶链反应（RT-PCR）技术检测了睾丸苗勒管抑制物质（MIS）的转录。通过这种非定量技术对睾丸进行的发育研究表明，在所有出生后阶段（包括成年期）都有表达，而肝脏和肾脏提供阴性对照。细胞分离研究表明，高度纯化的间质细胞，以及不太均匀的支持细胞富集和生殖细胞富集的馏分，含有RNA的MIS。在纯化的Leydig细胞中发现MIS mRNA，证实了MIS在间质细胞类型中的转录。由于生殖细胞富集部分含有一些支持细胞，XX，Sxr（a）和XX，Sxr（B）具有生殖细胞耗竭的睾丸，含有MIS mRNA，因此支持细胞来源可能仍然是曲细精小管隔室的来源。
We have examined the transcription of Mullerian inhibiting substance (MIS) in testis by the sensitive technique of reverse transcription polymerase chain reaction (RTPCR). A developmental study of testis by this nonquantitative technique showed expression at all postnatal stages, including adults while liver and kidney provided negative controls. Cell separation studies indicated that highly purified interstitial cells, as well as less homogeneous Sertoli cell-enriched and germ cell-enriched fractions, contained RNA for MIS. The transcription of MIS in an interstitial cell type was confirmed by finding MIS mRNA in purified Leydig cells. Inasmuch as the germ cell-enriched fraction contains some Sertoli cells, and XX,Sxr(a) and XX,Sxr(b) which have germ cell-depleted testes, contain MIS mRNA, a Sertoli cell source remains likely for the seminiferous tubule compartment.