Staphylococcus aureus enterotoxin B, protein A, and lipoteichoic acid stimulations in nasal polyps
Staphylococcus aureus enterotoxin B, protein A, and lipoteichoic acid stimulations in nasal polyps
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DOI：
10.1016/j.jaci.2007.08.059
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发表时间：
2008-01-01
影响因子：
14.2
通讯作者：
Bachert, Claus
中科院分区：
文献类型：
--
作者：
Patou, Joke;Gevaert, Philippe;Bachert, Claus
背景资料：越来越多的证据表明金黄色葡萄球菌及其产物在鼻息肉病的发病机制中具有修饰作用。目的：本研究的目的是研究鼻息肉和对照下鼻甲组织中金黄色葡萄球菌衍生蛋白肠毒素B、蛋白A和脂磷壁酸刺激后细胞因子和介质的产生。用RPMI（阴性对照）、肠毒素B、蛋白A和脂磷壁酸刺激组织碎片30分钟和24小时。通过多重测定上清液的促炎细胞因子（IL-1 β、TNF-α）和T细胞及亚群相关细胞因子（IFN-γ、IL-2、IL-4、IL-5、IL-8、IL-10、IL-12 p70、IL-13）。组胺，TGF-β 1，半胱氨酰白三烯，前列腺素D-2进行了分析，ELISA。结果：三十分钟的蛋白A刺激导致组胺，白三烯，前列腺素D-2的显着增加。肠毒素B刺激24小时后，两组患者的IL-10、TNF-α、IFN-γ、IL-2、IL-4、IL-5、IL-10和IL-13均显著增加，鼻息肉患者的增加显著高于对照组。表面蛋白A诱导肥大细胞脱颗粒，而肠毒素诱导细胞因子的释放，在鼻息肉中具有T（H）2-偏斜模式，支持超抗原在这种炎性疾病的发展中的刺激作用。
Background: Increasing evidence points toward a modifying role of Staphylococcus aureus and its products in the pathogenesis of nasal polyposis.Objective: The aim of this study was to investigate cytokine and mediator production after stimulation with S aureus-derived proteins enterotoxin B, protein A, and lipoteichoic acid in nasal polyp and control inferior turbinate tissue.Methods: Tissue fragments were stimulated with RPMI (negative control), enterotoxin B, protein A, and lipoteichoic acid for 30 minutes and 24 hours. Supernatants were measured by multiplex for proinflammatory cytokines (IL-1 beta, TNF-alpha) and T-cell and subset-related cytokines (IFN-gamma, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13). Histamine, TGF-beta 1, cysteinyl leukotrienes, and prostaglandin D-2 were analyzed by ELISA.Results: Thirty minutes of protein A stimulation resulted in a significant increase of histamine, leukotrienes, and prostaglandin D-2. Enterotoxin B stimulation over a period of 24 hours induced a significant increase of IL-10, TNF-alpha, IFN-gamma, IL-2, IL-4, IL-5, IL-10, and IL-13 in both groups, with this increase significantly higher in nasal polyps compared with controls.Conclusion: We here show that S aureus products have various effects on mucosal tissues: surface protein A induces mast cell degranulation, whereas enterotoxins induce the release of cytokines, with a T(H)2-skewed pattern in nasal polyps, supporting the stimulatory role of superantigens in the development of this inflammatory disease.