Semipersistent Whitefly Transmission of Squash vein yellowing virus, Causal Agent of Viral Watermelon Vine Decline
Semipersistent Whitefly Transmission of Squash vein yellowing virus, Causal Agent of Viral Watermelon Vine Decline
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DOI：
10.1094/pdis-09-11-0761
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发表时间：
2012-06-01
期刊：
影响因子：
4.5
通讯作者：
Reitz, Stuart R.
中科院分区：
文献类型：
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作者：
Webb, Susan E.;Adkins, Scott;Reitz, Stuart R.
Webb， S. E., Adkins， S.和Reitz， S. R. 2012。半持续性白蝇传播南瓜静脉黄变病毒，导致病毒性西瓜植株衰退。植物杂志。96:839-844。南瓜静脉变黄病毒（SqVYV）是最近发现的一种potyvirus科的Ipomovirus，是导致病毒性西瓜枯萎的原因，是佛罗里达州的一种毁灭性疾病。已知SqVYV是由白色烟粉虱（Gennadius） B株传播的，但以前没有调查传播过程的细节。我们完成了一系列实验，以确定传播效率，不同获取和接种时间的影响，白蝇保留传播病毒的时间长度，以及完成获取和接种周期所需的最短时间。效率很低，每个工厂至少需要30个白雪原才能持续传输。感染导致后来的传播在4小时达到高峰，接种时间超过4至8小时不会导致感染率增加。白蝇只保留病毒很短的时间，在离开受感染的植株后24小时内没有传播。在实验室条件下，完成一个传播周期至少需要3小时。这些结果证明了SqVYV的半持续性传播，并将有助于完善该病毒及其引起的疾病的流行病学模型。
Webb, S. E., Adkins, S., and Reitz, S. R. 2012. Semipersistent whitefly transmission of Squash vein yellowing virus, causal agent of viral watermelon vine decline. Plant Dis. 96:839-844.Squash vein yellowing virus (SqVYV), a recently described Ipomovirus sp. in the family Potyviridae, is the cause of viral watermelon vine decline, a devastating disease in Florida. SqVYV is known to be transmitted by the whitetly, Bemisia tabaci (Gennadius) B strain, but details of the transmission process have not previously been investigated. We completed a series of experiments to determine efficiency of transmission, effects of different acquisition and inoculation access periods, the length of time that whiteflies retained transmissible virus, and the minimum time needed to complete a cycle of acquisition and inoculation. Efficiency was low, with at least 30 whitetlies per plant needed for consistent transmission. Acquisition leading to later transmission peaked at 4 h, and inoculation access periods longer than 4 to 8 h led to no increase in infection rates. Whiteflies retained virus only a short time, with no transmission by 24 h after removal from infected plants. A minimum of 3 h was needed to complete a cycle of transmission under laboratory conditions. These results demonstrate semipersistent transmission of SqVYV and will help refine models of the epidemiology of this virus and the disease it causes.