Ion mobility-mass spectrometry as a tool to investigate protein-ligand interactions
Ion mobility-mass spectrometry as a tool to investigate protein-ligand interactions
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DOI：
10.1007/s00216-017-0384-9
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发表时间：
2017-07-01
影响因子：
4.3
通讯作者：
Pagel, Kevin
中科院分区：
文献类型：
--
作者：
Goeth, Melanie;Pagel, Kevin
离子迁移-质谱（IM-MS）是一种同时分析离子种类的质量、电荷、大小和形状的有力工具。它允许复杂样品中的低丰度物种的表征，因此特别适合于蛋白质及其组装体的分析。在过去的几年中，甚至复杂和棘手的物种已经成功地研究了与IM-MS和出版物的数量在这一领域正在稳步增长。这篇趋势文章强调了IM-MS用于研究蛋白质-配体复合物的最新进展，特别关注捕获和释放（CaR）策略和碰撞诱导的解折叠（CIU）。
Ion mobility-mass spectrometry (IM-MS) is a powerful tool for the simultaneous analysis of mass, charge, size, and shape of ionic species. It allows the characterization of even low-abundant species in complex samples and is therefore particularly suitable for the analysis of proteins and their assemblies. In the last few years even complex and intractable species have been investigated successfully with IM-MS and the number of publications in this field is steadily growing. This trend article highlights recent advances in which IM-MS was used to study protein-ligand complexes and in particular focuses on the catch and release (CaR) strategy and collision-induced unfolding (CIU).