Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti
Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti
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DOI：
10.1111/j.1365-3024.2009.01155.x
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发表时间：
2009-12-01
影响因子：
2.2
通讯作者：
Rohousova, I.
中科院分区：
文献类型：
--
作者：
Drahota, J.;Lipoldova, M.;Rohousova, I.
沙蝇是一种吸血昆虫，传播利什曼原虫属寄生虫，利什曼原虫是人类和狗疾病的病原体。实验宿主反复接触沙蝇唾液可控制利什曼原虫感染。细胞介导的抗唾液免疫反应很可能是这种保护作用的原因；然而，到目前为止，还没有关于其对不同沙蝇载体的抗原特异性的研究。本研究用来自三种不同沙蝇载体的唾液腺匀浆体外攻击反复暴露于白纹伊蚊叮咬的BALB/c小鼠的脾细胞。Sergenti、P.Papatasi或P.arabicus。Sergenti咬伤小鼠对同种抗原的增殖反应高于幼龄小鼠脾细胞。瑟氏疟原虫叮咬小鼠的脾细胞和抗P。Sergenti抗体与巴氏杆菌唾液发生部分交叉反应。相反，未发现与阿拉伯巴氏杆菌唾液的交叉反应。我们的数据表明，免疫系统的两个手臂，细胞和体液，都以物种特有的方式做出反应。因此，针对某一物种唾液成分的抗体的存在也表明了细胞介导的免疫反应的特异性。这些数据表明，每种媒介-利什曼原虫组合都需要独特的传播阻断疫苗。
P>Sand flies are bloodsucking insects transmitting parasites of genus Leishmania, the causative agents of diseases in humans and dogs. Experimental hosts repeatedly exposed to sand fly saliva can control Leishmania infection. Cell-mediated anti-saliva immune response is most likely responsible for this protective effect; however, there is no study so far concerning its antigenic specificity towards different sand fly vectors. In this study, splenocytes from BALB/c mice repeatedly exposed to the bites of Phlebotomus sergenti were challenged ex vivo with salivary gland homogenates from three different sand fly vectors -P. sergenti, P. papatasi, or P. arabicus. Mice bitten by P. sergenti had higher proliferative response to homologous antigen than splenocytes from naive mice. Splenocytes from P. sergenti bitten mice as well as anti-P. sergenti antibodies partially cross-reacted with P. papatasi saliva. In contrast, no cross-reactivity was found with P. arabicus saliva. Our data indicate that both arms of the immune system, cellular and humoral, react in a species-specific manner. Therefore, the presence of antibodies against salivary components of a certain species indicates the specificity of cell-mediated immune response as well. The data suggest that unique transmission-blocking vaccine would be required for each vector -Leishmania combination.