Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption
Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption
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DOI：
10.1096/fj.13-231860
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发表时间：
2013-09-01
期刊：
影响因子：
4.8
通讯作者：
Jia, Wei
中科院分区：
文献类型：
--
作者：
Xie, Guoxiang;Zhong, Wei;Jia, Wei
我们对胆汁酸代谢的理解受到以下事实的限制，即先前的分析主要集中在选定的几种循环胆汁酸;很少研究肝脏和胃肠道池的胆汁酸谱。在这里，我们确定了慢性乙醇消耗如何改变大鼠多个身体隔室（肝脏，胃肠道和血清）中的胆汁酸。给大鼠喂食改良的Lieber-DeCarli液体饮食，其中38%的卡路里为乙醇（相当于人类4-5杯饮料的量）。虽然结合胆汁酸主要存在于肝脏（98.3%）、十二指肠（97.8%）和回肠（89.7%）中，但非结合胆汁酸在血清（81.2%）、盲肠（97.7%）和直肠（97.5%）中所占比例最大。特别是，牛磺酸结合胆汁酸显着减少，在乙醇处理的大鼠的肝脏和胃肠道，而非共轭和甘氨酸共轭的物种增加。乙醇消耗导致参与胆汁酸生物合成，外排转运的基因表达增加，调节肝脏胆汁酸内流转运的基因表达减少。这些结果提供了对哺乳动物中胆汁酸代谢通过肠-肝轴的系统性调节的更好理解。钟伟，Li，H.，李，Q.，Qiu，Y.，中国农业大学出版社，郑，X.，陈洪，赵，X.，Zhang，S.，（1991），中国农业科学院，周志，蔡塞尔，S. H、贾，W.慢性乙醇消耗引起大鼠胆汁酸代谢的改变。
Our understanding of the bile acid metabolism is limited by the fact that previous analyses have primarily focused on a selected few circulating bile acids; the bile acid profiles of the liver and gastrointestinal tract pools are rarely investigated. Here, we determined how chronic ethanol consumption altered the bile acids in multiple body compartments (liver, gastrointestinal tract, and serum) of rats. Rats were fed a modified Lieber-DeCarli liquid diet with 38% of calories as ethanol (the amount equivalent of 4-5 drinks in humans). While conjugated bile acids predominated in the liver (98.3%), duodenum (97.8%), and ileum (89.7%), unconjugated bile acids comprised the largest proportion of measured bile acids in serum (81.2%), the cecum (97.7%), and the rectum (97.5%). In particular, taurine-conjugated bile acids were significantly decreased in the liver and gastrointestinal tract of ethanol-treated rats, while unconjugated and glycine-conjugated species increased. Ethanol consumption caused increased expression of genes involved in bile acid biosynthesis, efflux transport, and reduced expression of genes regulating bile acid influx transport in the liver. These results provide an improved understanding of the systemic modulations of bile acid metabolism in mammals through the gut-liver axis.Xie, G., Zhong, W., Li, H., Li, Q., Qiu, Y., Zheng, X., Chen, H., Zhao, X., Zhang, S., Zhou, Z., Zeisel, S. H., Jia, W. Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption.