Electron microscope studies of epidermal melanocytes, and the fine structure of melanin granules.
Electron microscope studies of epidermal melanocytes, and the fine structure of melanin granules.
复制标题
表皮黑素细胞的电子显微镜研究和黑色素颗粒的精细结构。
DOI：
10.1083/jcb.8.1.165
复制
发表时间：
1960-09
期刊：
影响因子：
--
通讯作者：
DROCHMANS, P
中科院分区：
文献类型：
--
作者：
DROCHMANS, P
用电子显微镜研究了正常人和猫皮肤以及增生性人类皮肤病变中的黑素细胞和黑色素颗粒。黑素细胞一直以游离细胞的形式存在于真皮内，即真皮膜的表皮一侧。黑素细胞通常停留在真皮膜上或向真皮突起。在这种情况下，连续的真皮膜看起来均匀薄而光滑，与马氏细胞旁边的区域形成对比，在那里它看起来明显更厚，似乎锚定在基底细胞层上。根据黑色素颗粒在黑素细胞中的位置和在颗粒成熟过程中可能只表现不同阶段的形态特征，将黑色素颗粒分为两种：(A)浅色黑色素颗粒，其结构类似于细小的网状结构；(B)致密黑色素颗粒，在臭氧固定的制剂中，呈现为均匀致密的团块，周围环绕着粗粒状、强烈嗜奥米性的外壳。用高锰酸钾处理组织切片后发现，在致密的颗粒中，存在规则晶格形式的有组织的骨架。据推测，这种基本结构本质上是蛋白质，可能包括能够产生黑色素的酶系统。据报道，在黑素细胞的细胞质中存在细丝，在形态上与马氏细胞中发现的张力细丝不同。
Melanocytes and melanin granules have been studied by electron microscopy in normal human and cat skin, and in hyperplastic human skin lesions. The melanocytes have always been found as free cells within the epidermis,i.e., on the epidermal side of the dermal membrane. Melanocytes frequently rest on the dermal membrane or bulge towards the dermis. In such cases the uninterrupted dermal membrane is uniformly thin and smooth in appearance, in contrast with the regions alongside Malpighian cells, where it appears appreciably thicker and seemingly anchored to the basal cell layer. Two types of melanin granules have been distinguished according to their location in the melanocytes and to morphological characteristics which may only express different stages in the maturation of the granules: (a) light melanin granules in which a structure resembling a fine network is apparent; (b) dense melanin granules which, in osmium-fixed preparations, appear as uniformly dense masses surrounded by a coarsely granular, intensely osmiophilic shell. Treatment of sections of osmium-fixed tissues with potassium permanganate has revealed within the dense granules the existence of an organized framework in the form of a regular, crystalline-like lattice. It is suggested that this basic structure is protein in nature and may include the enzymatic system capable of producing melanin. The existence is reported of fine filaments located in the cytoplasm of melanocytes and morphologically distinct from the tonofilaments found in Malpighian cells.