Alteration of nuclear protein profiling for NIH-3T3 cells exposed to H2O2
Alteration of nuclear protein profiling for NIH-3T3 cells exposed to H2O2
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暴露于 H2O2 的 NIH-3T3 细胞核蛋白谱的变化
DOI：
10.1002/cbf.1693
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发表时间：
2010-10-01
影响因子：
3.6
通讯作者：
Zhao, Liang
中科院分区：
文献类型：
--
作者：
Liu, Yawei;He, Minyi;Zhao, Liang
氧化应激对哺乳动物细胞构成威胁。为了更好地了解氧化应激背后的分子反应和机制，我们应用二维聚丙烯酰胺凝胶电泳和基质辅助激光解吸电离战斗时间质谱分析来鉴定暴露于温和 H2O2 的小鼠成纤维细胞 NIH-3T3 细胞的差异核蛋白谱。 MS 鉴定出 13 种差异表达蛋白，其中 2 种通过 Western blot 进一步验证。结果显示，暴露于温和的H2O2 12小时导致DJ-1、谷胱甘肽S-转移酶P 1、DNA连接酶I、动力蛋白2、核磷蛋白的表达上调，以及核苷二磷酸激酶A、烯醇化酶-a、自整合屏障因子1、转移相关蛋白1、含有糖基转移酶样结构域的蛋白1、联会蛋白的表达下调复合蛋白 1、α-centtractin、溴结构域和含有 PHD 手指 1 (BRPF1)。先前的研究支持大多数已识别的蛋白质是定位的核蛋白质。这些发现可能为阐明细胞对 H2O2 的反应以及成纤维细胞对抗氧化应激的潜在机制提供一些线索。版权所有 (C) 2010 约翰·威利父子有限公司
Oxidative stress is a threat to mammalian cells. To better understand the molecular response and mechanism underlying oxidative stress, we applied two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-fight mass spectrometry analysis to identify differential nuclear protein profiling of mouse fibroblast NIH-3T3 cells exposed to mild-H2O2. Thirteen differentially expressed proteins were identified by MS and two of them were further validated by Western blot. The results revealed that exposure to mild-H2O2 for 12 h cause up-regulated expression of DJ-1, glutathione S-transferase P 1, DNA ligase I, dynamin 2, nucleophosmin, and down-regulated expression of nucleoside diphosphate kinase A, enolase-a, barrier-to-autointegration factor 1, metastasis associated protein 1, glycosytransferase-like domain containing protein 1, synaptonemal complex protein 1, alpha-centractin, bromodomain, and PHD finger containing 1 (BRPF1). Most of the identified proteins are supported as nuclear proteins localized by previous research. The findings may provide some clues to elucidate cell responses to H2O2 and the potential mechanism underlying protection against oxidative stress in fibroblast cells. Copyright (C) 2010 John Wiley & Sons, Ltd.