Aquaporin expression in the brains of patients with or without cerebral amyloid angiopathy.
Aquaporin expression in the brains of patients with or without cerebral amyloid angiopathy.
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DOI：
10.1097/nen.0b013e3181fd252c
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发表时间：
2010-12
影响因子：
3.2
通讯作者：
Vinters HV
中科院分区：
文献类型：
--
作者：
Moftakhar P;Lynch MD;Pomakian JL;Vinters HV
水通道蛋白是近年来发现的参与水转运的蛋白质通道。这些通道可能在阿尔茨海默病（AD）和脑淀粉样血管病（CAA）中观察到的水肿形成和微血管功能改变中发挥作用。应用免疫组化方法检测了24例尸检脑组织水通道蛋白1（AQP 1）和水通道蛋白4（AQP 4）的表达，其中18例为AD患者，6例为无病理改变的CAA患者。与年龄和性别匹配的对照组相比，AD和CAA患者的AQP 4表达增强。水通道蛋白4免疫反应性在CSF和脑界面，包括软膜下，室管膜下，毛细血管周围和动脉周围空间突出。水通道蛋白1在AD和CAA病例中的表达与年龄和性别匹配的对照组没有差异。双标记研究表明，AQP1和4都定位于星形胶质细胞。增强的AQP4表达和其独特的染色模式表明，这些蛋白质可能是重要的，在AD和CAA中观察到的受损的水转运。
Aquaporins have recently been identified as protein channels involved in water transport. These channels may play a role in the edema formation and alterations in microvascular function observed in Alzheimer disease (AD) and cerebral amyloid angiopathy (CAA). We investigated the expression of aquaporin 1 (AQP1) and aquaporin 4 (AQP 4) in 24 human autopsy brains consisting of 18 with AD and varying degrees of CAA, and 6 with no pathologic abnormalities using immunohistochemistry. In cases of AD and CAA there was enhanced AQP 4 expression compared to age- and gender-matched controls. Aquaporin 4 immunoreactivity was prominent at CSF and brain interfaces, including subpial, subependymal, pericapillary and periarteriolar spaces. Aquaporin 1 expression in AD and CAA cases was not different from that in age- and gender-matched controls. Double-labeling studies demonstrated that both AQP1 and 4 were localized to astrocytes. Both enhanced AQP4 expression and its unique staining pattern suggest that these proteins may be important in the impaired water transport observed in AD and CAA.