A multicolor electrochemiluminescence device based on closed bipolar electrode for rapid visual screening of Salmonella typhimurium
A multicolor electrochemiluminescence device based on closed bipolar electrode for rapid visual screening of Salmonella typhimurium
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基于封闭双极电极的多色电化学发光装置快速目视筛查鼠伤寒沙门氏菌
DOI：
10.1016/j.snb.2021.130761
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发表时间：
2021-09-29
影响因子：
8.4
通讯作者：
Xiong, Xiaohui
中科院分区：
文献类型：
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作者：
Luo, Yun;Lv, Fenglan;Xiong, Xiaohui
建立了一种基于封闭双极电极（BPE）的多色电化学发光（ECL）装置，用于鼠伤寒沙门氏菌（S. typhimurium）可视化检测。由于伴随的电化学发光团([Ir（py)3]和[Ru(py)3]2+）的发射颜色是有可能区分的，因此可以通过调节BPE两极的界面电位来获得ECL的选择性激发。通过调节BPE的电阻，在阳极处可以观察到从深橙色到黄色到森林绿色的双向颜色变化，这归因于伴随的电化学发光团的双电位激发特性。鼠伤寒沙门氏菌在BPE阴极上的存在增加了电路中的电阻，在另一极开关处产生ECL报告颜色。根据该多色BPE-ECL传感器，25 mL (g)样品中鼠伤寒沙门氏菌的裸眼检出限为10 CFU mL-1。在这项工作中，封闭BPE的阴极和阳极的物理隔离不仅避免了靶和电化学发光团的相互干扰，而且BPE的表面不需要任何修饰，只需要添加免疫磁珠(IMBs)-S。鼠伤寒化合物到阴极。此外，无需复杂的预富集细菌，整个实验时间也可大大缩短，仅需0.5 h。另一方面，将电化学信号转换为更直观的光学读数，用于食品中鼠伤寒沙门氏菌的可视化传感。该多色ECL装置将电化学的灵敏度与光学读数的直观性相结合，可应用于食品安全的快速检测现场。
A multicolor electrochemiluminescence (ECL) device was constructed based on closed bipolar electrode (BPE) for visualized sensing of Salmonella typhimurium (S. typhimurium). Since the emission color of concomitant electrochemiluminophores ([Ir(ppy)3] and [Ru(bpy)3]2+) is potentially distinguish, selective excitation of ECL could be obtained by regulating the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, bidirectional color change from dark-orange to yellow to forest-green could be observed at the anode, which was ascribed to the dual-potential excitation property of the concomitant electrochemiluminophores. The presence of S. typhimurium on the cathode of BPE increases the resistance in the circuit, generating ECL reporting color at the other pole switched. In accordance with this multicolor BPE-ECL sensor, a low detection limit of 10 CFU mL-1 of S. typhimurium could be identified in 25 mL (g) sample with naked eyes. In this work, physical isolation of the cathode and anode of closed BPE not only avoids target and electrochemiluminophores mutual interference, but also the surface of the BPE need nothing modified, just added immunomagnetic beads (IMBs)-S. typhimurium compound to the cathode. Moreover, without complex pre-enrichment bacterial, the whole experiment time also can be greatly shortened with only 0.5 h. On the other hand, the electrochemical signal is converted into a more intuitive optical readout for visualized sensing of S. typhimurium in food. The multicolor ECL device is merging the sensitivity of electrochemistry with the intuitive of an optical readout, which may apply in the site of rapid detection of food safety.