Molecular mapping of the potato virus Y resistance gene Ry(sto) in potato
Molecular mapping of the potato virus Y resistance gene Ry(sto) in potato
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DOI：
10.1007/s001220050400
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发表时间：
1997-02-01
影响因子：
5.4
通讯作者：
Baulcombe, DC
中科院分区：
文献类型：
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作者：
Brigneti, G;GarciaMas, J;Baulcombe, DC
Ry（sto）基因是马铃薯抗马铃薯Y病毒（PVY）的显性基因。我们利用F-1四倍体马铃薯群体的混合分离分析，确定了与Ry（sto）连锁的三个AFLP标记。这些AFLP标记之一的番茄同源物被分配到连锁群XI通过分析番茄的F-2作图群体，表明Ry（sto）也是在染色体XI的马铃薯基因组。通过证明Ry（sto）与先前已定位于马铃薯基因组的染色体XI的标记连锁，证实了该图谱位置。在抗性（Ry（sto））和感病亲本之间的马铃薯杂交的360个分离后代的群体中鉴定了与Ry（sto）紧密连锁的另外4个AFLP标记。这些标记中的两个位于Ry（sto）的两侧，仅由单个重组事件分开。另外两个标记与Ry（sto）共分离。
Ry(sto) is a dominant gene which confers resistance to potato virus Y (PVY) in potato. We have used bulked segregant analysis of an F-1 tetraploid potato population to identify three AFLP markers linked to and on either side of Ry(sto). The tomato homologue of one of these AFLP markers was assigned to linkage group XI by analysis of an F-2 mapping population of tomato, suggesting that Ry(sto) is also on chromosome XI of the potato genome. This map position was confirmed by the demonstration that Ry(sto) was linked to markers which had been previously mapped to chromosome XI of the potato genome. Four additional AFLP markers were identified that were closely linked to Ry(sto) in a population of 360 segregating progeny of a potato cross between a resistant (Ry(sto)) and a susceptible parent. Two of these markers were on either side of Ry(sto), separated by only a single recombination event. The other two markers co-segregated with Ry(sto).