Transcriptome comparison reveals key candidate genes in response to vernalization of Oriental lily.
Transcriptome comparison reveals key candidate genes in response to vernalization of Oriental lily.
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转录组比较揭示了响应东方百合春化的关键候选基因
DOI：
10.1186/s12864-016-2955-0
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发表时间：
2016-08-22
期刊：
影响因子：
4.4
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Li W;Liu X;Lu Y
东方百合‘Sorbonne’是百合的重要切花，在世界范围内广受欢迎，但它必须经历一段较低的冬季气温才能启动或加速开花过程。为了更好地了解温度信号通路和春化反应中涉及的分子代谢反应，使用RNA-Seq进行了全基因组转录分析。188,447,956个测序读数组装成66,327个非基因，与Swiss-Prot蛋白质数据库中的已知蛋白质相似，2,893，30,406和60,737个非基因与KEGG，COG和GO数据库中的现有序列比对。基于qRT-PCR结果，我们研究了植物激素信号转导(LoAP2、LoIAA1、LoARF10)、DNA甲基化(LoCMT、LoFLD)和春化蛋白途径(LoFLC、LoVRN1、LoVRN2、LOFT、LoSOC1、LoLFY、LoSVP)三个信号调节通路基因在东方百合未成熟花蕾中的表达。此外，我们还从文库中筛选到两个春化相关基因(LoSVP和LoVRN1)，它们可能在东方百合的开花发育和开花反应中起关键作用，LoSVP具有延缓开花的功能，而LoVRN1具有促进早花的功能。我们采集了鳞茎顶端分生组织处于花的过渡时期，而顶端分生组织尚未转化为形态分化过程时的转录组进行测序和比较，这有助于获得更多在花诱导途径中起关键作用的基因。通过分析基因在大范围内的表达水平，预测了不同基因之间的上下游关系。未来针对基因如何相互作用的研究，将有助于建立和完善春化诱导成花途径的分子机制。
Oriental hybrid lily ‘Sorbonne’, a very important cut flower for lily, is enjoyed great popularity in the world, but it must experience a period of low winter temperature to initiate or accelerate the flowering process. To gain a better understanding of the temperature signaling pathway and the molecular metabolic reactions involved in the vernalization response, a genome-wide transcriptional analysis using RNA-Seq was performed. 188,447,956 sequencing reads was assembled into 66,327 unigenes and showed similarity to known proteins in the Swiss-Prot protein database, and 2,893, 30,406 and 60,737 unigenes aligned to existing sequences in the KEGG, COG, and GO databases. Based on qRT-PCR results, we studied the expression of three signal regulation pathways genes–the plant hormones signal transduction (LoAP2, LoIAA1, LoARF10), the DNA methylation (LoCMT, LoFLD), and vernalizatin pathway (LoFLC, LoVRN1, LoVRN2, LoFT, LoSOC1, LoLFY, LoSVP) in the immature flower buds of Oriental hybrid lily. In addition, we identified two vernalizaiton–related genes (LoSVP and LoVRN1) from the cDNA library, which appear to be promising candidates for playing key roles in the development and response of flowering in Oriental lily plants, and LoSVP had a function in delaying flowering but LoVRN1could promote flowering early. We collected a sample for transcriptome sequencing and comparison when the bulb’s apical meristem was in the time of floral transition when the apical meristem had not converted into the morphological differentiation process, which helped to obtain more genes playing key roles in the floral induction pathways. The upstream and downstream relationship between different genes were forecasted by the analysis of genes’ expression levels in a wide range of time. Future research that is targeted towards how genes interact on each other, which will promote establishing and perfecting the molecular mechanisms of floral induction pathway by vernalization.