Voltage-sensitive dye recording of glossopharyngeal nerve-related synaptic networks in the embryonic mouse brainstem.
Voltage-sensitive dye recording of glossopharyngeal nerve-related synaptic networks in the embryonic mouse brainstem.
复制标题
胚胎小鼠脑干中舌咽神经相关突触网络的电压敏感染料记录。
DOI：
10.1016/j.ibror.2019.05.004
复制
发表时间：
2020
期刊：
影响因子：
2.6
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y. and Sato;K.
舌咽神经（N.IX）传递与内脏和躯体功能相关的运动和感觉信息，如唾液分泌，味觉和血压控制。N. IX相关的神经回路对于这些基本功能是不可或缺的。与形态发生的艰苦分析相比，我们才刚刚开始阐明这些神经回路在个体发育过程中的功能。在本研究中，我们应用电压敏感染料记录胚胎小鼠脑干，并检查了N. IX相关神经回路的功能发育。首先，我们光学鉴定了运动核（下唾液核（ISN））和一级感觉核（孤束核（NTS））。我们还成功地记录光学反应的第二/高阶感觉核通过NTS，包括臂旁核。第二，我们在N. IX相关核团中追踪神经元兴奋性和突触功能的起始。ISN中的神经元至少在E11时是可兴奋的，NTS中的功能性突触传递在E12时首先表达。在第二/高阶感觉核中，突触功能在E12-13左右出现。第三，通过绘制对N.IX和迷走神经（N.X）刺激的光学反应，我们发现，从个体发育的早期开始，N.IX和N.X在NTS中的神经活动分布模式是不同的。我们讨论了N. IX相关的神经回路的形成在脑干中，与我们以前的结果从鸡和大鼠胚胎。
The glossopharyngeal nerve (N.IX) transfers motor and sensory information related to visceral and somatic functions, such as salivary secretion, gustation and the control of blood pressure. N.IX-related neural circuits are indispensable for these essential functions. Compared with the strenuous analysis of morphogenesis, we are only just starting to elucidate the functiogenesis of these neural circuits during ontogenesis. In the present study, we applied voltage-sensitive dye recording to the embryonic mouse brainstem, and examined the functional development of the N.IX-related neural circuits. First, we optically identified the motor nucleus (the inferior salivatory nucleus (ISN)) and the first-order sensory nucleus (the nucleus of the tractus solitarius (NTS)). We also succeeded in recording optical responses in the second/higher-order sensory nuclei via the NTS, including the parabrachial nucleus. Second, we pursued neuronal excitability and the onset of synaptic function in the N.IX-related nuclei. The neurons in the ISN were excitable at least at E11, and functional synaptic transmission in the NTS was first expressed at E12. In the second/higher-order sensory nuclei, synaptic function emerged at around E12-13. Third, by mapping optical responses to N.IX and vagus nerve (N.X) stimulation, we showed that the distribution patterns of neural activity in the NTS were different between the N.IX and the N.X from the early stage of ontogenesis. We discuss N.IX-related neural circuit formation in the brainstem, in comparison with our previous results obtained from chick and rat embryos.