Field deposition of Bt transgenic corn pollen: lethal effects on the monarch butterfly
Field deposition of Bt transgenic corn pollen: lethal effects on the monarch butterfly
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DOI：
10.1007/s004420000502
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发表时间：
2000-10-01
期刊：
影响因子：
2.7
通讯作者：
Obrycki, JJ
中科院分区：
文献类型：
--
作者：
Jesse, LCH;Obrycki, JJ
我们首次提出了转基因苏云金芽孢杆菌(Bt)玉米花粉自然寄生在黑星天牛上的证据；玉米田中的普通马利筋能显著地导致斑潜蝇幼虫的死亡。取食含Bt玉米花粉的黑麦草幼虫48h死亡率显著高于取食无花粉叶片的幼虫(3+/-3%)和取食非Bt花粉叶片的幼虫(0%)。135粒/cm2的转基因花粉作用48 h后，棉铃虫幼虫120 h的死亡率为37%~70%。我们发现，在三种浓度的Bt花粉中暴露48小时后，对从幼虫饲养的丛枝金龟子成虫没有亚致死效应。根据我们对这种花粉在农田边缘以外的风传播的量化，我们预测转基因花粉对丛枝金龟子的影响可能发生在距离转基因农田边界至少10m的地方，然而，最高的幼虫死亡率可能发生在玉米田的黑粉虫植株上或转基因玉米田边缘的3m以内。我们的结论是，在大面积种植转基因杀虫作物之前，需要对其生态效应进行更全面的评估。
We present the first evidence that transgenic Bacillus thuringiensis (Bt) corn pollen naturally deposit ed on Asclepias syriaca; common milkweed, in a corn field causes significant mortality of Danaus plexippus L. (Lepidoptera: Danaidae) larvae. Larvae feeding for 48 h on A. syriaca plants naturally dusted with pollen from Bt corn plants suffered significantly higher rates of mortality at 48 h (20+/-3%) compacted to larvae feeding on leaves with no pollen (3+/-3%), or feeding on leaves with non-Bt pollen (0%). Mortality at 120 h of D. plexippus larvae exposed to 135 pollen grains/cm(2) of transgenic pollen for 48 h ranged from 37 to 70%. We found no sub-lethal effects on D. plexippus adults reared from larvae that survived a 48-h exposure to three concentrations of Bt pollen. Based on our quantification of the wind dispersal of this pollen beyond the edges of agricultural fields, we predict that the effects of transgenic pollen on D. plexippus may be observed at least 10 m from transgenic field borders, However, the highest larval mortality will likely occur on A. syriaca plants in corn fields or within 3 m of the edge of a transgenic corn field. We conclude that the ecological effects of transgenic insecticidal crops need to be evaluated more fully before they are planted over extensive areas.