The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.
The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.
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DOI：
10.1002/j.1460-2075.1990.tb08258.x
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发表时间：
1990-05
期刊：
影响因子：
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通讯作者：
P. Reynolds;Marcel H.M. Koken;Jan H. J. Hoeijmakers;S. Prakash;L. Prakash
中科院分区：
文献类型：
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作者：
P. Reynolds;Marcel H.M. Koken;Jan H. J. Hoeijmakers;S. Prakash;L. Prakash
酿酒酵母的RAD 6基因编码泛素结合酶，并且是DNA修复、DNA损伤诱导的诱变和孢子形成所需的。在这里，我们表明，RAD 6和rhp 6+基因从远亲酵母裂殖酵母共享高度的结构和功能同源性。RAD 6蛋白中主要存在的酸性羧基末端21个氨基酸在rhp 6（+）编码的蛋白中不存在;除此之外，这两种蛋白非常相似，具有77%相同的残基。与rad 6一样，rhp 6+基因的无效突变导致DNA修复、UV诱变和孢子形成缺陷，并且RAD 6和rhp 6+基因可以在功能上相互取代。这些观察结果表明，RAD 6（rhp 6+）蛋白和DNA修复复合物的其他组件之间的功能相互作用已经在真核生物中保守。
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enzyme and is required for DNA repair, DNA‐damage‐induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxyl‐terminal 21 amino acids present in the RAD6 protein are absent in the rhp6(+)‐encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.